The aim of the study was to compare the metabolic activity of brain cortex after the acute hypoxia caused by the impairment of breathing or blood circulation. Male Wistar rats were randomized in two groups: impaired breathing and blood circulation failure groups. Fluorescence under 365 and 450 nm excitation and diffuse reflectance intensity at 550-820 nm range were estimated. We found that after long-term hypoxic conditions, notable metabolic changes occur. We suppose that oxygen deficiency causes an activation of the GABA shunt mechanism. In cases of blood circulation failure, fluorescence intensity changes faster than in cases of breathing impairment.
|Journal||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Publication status||Published - 21 Feb 2020|
|Event||Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis 2020 - San Francisco, United States|
Duration: 2 Feb 2020 → 5 Feb 2020
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- Brain metabolism
- Diffuse reflectance spectra
- Fluorescence spectroscopy
- Laser speckle contrast imaging
- Neuronal hypoxia
- Resuscitation procedures