TY - JOUR
T1 - Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells
AU - Kitchen, Philip
AU - Salman, Mootaz M
AU - Abir-Awan, Mohammed
AU - Al-Jubair, Tamim
AU - Törnroth-Horsefield, Susanna
AU - Conner, Alex C
AU - Bill, Roslyn M
N1 - © 2020 The Author(s).
PY - 2020/12/18
Y1 - 2020/12/18
N2 - Aquaporins (AQPs) are membrane channel proteins that facilitate the movement of water down osmotic gradients across biological membranes. This protocol allows measurements of AQP-mediated water transport across the plasma membrane of live mammalian cells. Calcein is a fluorescent dye that is quenched in a concentration-dependent manner. Therefore, on short timescales, its concentration-dependent fluorescence can be used as a probe of cell volume, and therefore a probe of water transport into or out of cells. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Kitchen and Conner (2015). For the underlying methodology development, please refer to Fenton et al. (2010) and Solenov et al. (2004).
AB - Aquaporins (AQPs) are membrane channel proteins that facilitate the movement of water down osmotic gradients across biological membranes. This protocol allows measurements of AQP-mediated water transport across the plasma membrane of live mammalian cells. Calcein is a fluorescent dye that is quenched in a concentration-dependent manner. Therefore, on short timescales, its concentration-dependent fluorescence can be used as a probe of cell volume, and therefore a probe of water transport into or out of cells. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Kitchen and Conner (2015). For the underlying methodology development, please refer to Fenton et al. (2010) and Solenov et al. (2004).
UR - https://www.sciencedirect.com/science/article/pii/S2666166720301441?via%3Dihub
U2 - 10.1016/j.xpro.2020.100157
DO - 10.1016/j.xpro.2020.100157
M3 - Article
C2 - 33377051
VL - 1
IS - 3
M1 - 100157
ER -