CD4+ T cell surface alpha enolase is lower in older adults

Stuart J. Bennett, Edyta M. Augustyniak, Christopher R. Dunston, Richard A. Brown, Eduard Shantsila, Gregory Y.H. Lip, Rita D.C. Torrão, Chathyan Pararasa, Ali R. Hussein, Romain Ladouce, Bertrand Friguet, Helen R. Griffiths*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4+ T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25y) and older (n=10; 50-70y) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4+ T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4+ T cells (p<0.05). In an independent age-matched case-control study, lower CD4+ T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.

Original languageEnglish
Pages (from-to)56–62
Number of pages7
JournalMechanisms of Ageing and Development
Volume152
Early online date1 Oct 2015
DOIs
Publication statusPublished - Dec 2015

Bibliographical note

© 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Funding: MARK-AGE (EU FP7 Large-scale integrating Project HEALTH-F4-2008-200880) and COST CM1001.

Supplementary data avaialbe on the jornal website.

Keywords

  • enolase
  • phenotype
  • 2D gel electrophoresis
  • CD4+T cell
  • plasma membrane proteomics

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    Bennett, S. J., Augustyniak, E. M., Dunston, C. R., Brown, R. A., Shantsila, E., Lip, G. Y. H., Torrão, R. D. C., Pararasa, C., Hussein, A. R., Ladouce, R., Friguet, B., & Griffiths, H. R. (2015). CD4+ T cell surface alpha enolase is lower in older adults. Mechanisms of Ageing and Development, 152, 56–62. https://doi.org/10.1016/j.mad.2015.09.005