CD4+ T cell surface alpha enolase is lower in older adults

Stuart J. Bennett, Edyta M. Augustyniak, Christopher R. Dunston, Richard A. Brown, Eduard Shantsila, Gregory Y.H. Lip, Rita D.C. Torrão, Chathyan Pararasa, Ali R. Hussein, Romain Ladouce, Bertrand Friguet, Helen R. Griffiths

Research output: Contribution to journalArticle

Abstract

To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4+ T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25y) and older (n=10; 50-70y) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4+ T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4+ T cells (p<0.05). In an independent age-matched case-control study, lower CD4+ T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.

LanguageEnglish
Pages56–62
Number of pages7
JournalMechanisms of Ageing and Development
Volume152
Early online date1 Oct 2015
DOIs
Publication statusPublished - Dec 2015

Fingerprint

Phosphopyruvate Hydratase
T-Lymphocytes
Young Adult
Proteins
Cell Aging
Electrophoresis, Gel, Two-Dimensional
Proteome
Vascular Diseases
Liquid Chromatography
Case-Control Studies
Mass Spectrometry
Flow Cytometry
Membrane Proteins
Cardiovascular Diseases
Age Groups
Cell Membrane
Messenger RNA
Membranes

Bibliographical note

© 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Funding: MARK-AGE (EU FP7 Large-scale integrating Project HEALTH-F4-2008-200880) and COST CM1001.

Supplementary data avaialbe on the jornal website.

Keywords

  • enolase
  • phenotype
  • 2D gel electrophoresis
  • CD4+T cell
  • plasma membrane proteomics

Cite this

Bennett, S. J., Augustyniak, E. M., Dunston, C. R., Brown, R. A., Shantsila, E., Lip, G. Y. H., ... Griffiths, H. R. (2015). CD4+ T cell surface alpha enolase is lower in older adults. Mechanisms of Ageing and Development, 152, 56–62. https://doi.org/10.1016/j.mad.2015.09.005
Bennett, Stuart J. ; Augustyniak, Edyta M. ; Dunston, Christopher R. ; Brown, Richard A. ; Shantsila, Eduard ; Lip, Gregory Y.H. ; Torrão, Rita D.C. ; Pararasa, Chathyan ; Hussein, Ali R. ; Ladouce, Romain ; Friguet, Bertrand ; Griffiths, Helen R. / CD4+ T cell surface alpha enolase is lower in older adults. In: Mechanisms of Ageing and Development. 2015 ; Vol. 152. pp. 56–62.
@article{3aea9435203f4691b39f27ea9a45c07b,
title = "CD4+ T cell surface alpha enolase is lower in older adults",
abstract = "To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4+ T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25y) and older (n=10; 50-70y) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4+ T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4+ T cells (p<0.05). In an independent age-matched case-control study, lower CD4+ T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.",
keywords = "enolase, phenotype, 2D gel electrophoresis, CD4+T cell, plasma membrane proteomics",
author = "Bennett, {Stuart J.} and Augustyniak, {Edyta M.} and Dunston, {Christopher R.} and Brown, {Richard A.} and Eduard Shantsila and Lip, {Gregory Y.H.} and Torr{\~a}o, {Rita D.C.} and Chathyan Pararasa and Hussein, {Ali R.} and Romain Ladouce and Bertrand Friguet and Griffiths, {Helen R.}",
note = "{\circledC} 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Funding: MARK-AGE (EU FP7 Large-scale integrating Project HEALTH-F4-2008-200880) and COST CM1001. Supplementary data avaialbe on the jornal website.",
year = "2015",
month = "12",
doi = "10.1016/j.mad.2015.09.005",
language = "English",
volume = "152",
pages = "56–62",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier",

}

Bennett, SJ, Augustyniak, EM, Dunston, CR, Brown, RA, Shantsila, E, Lip, GYH, Torrão, RDC, Pararasa, C, Hussein, AR, Ladouce, R, Friguet, B & Griffiths, HR 2015, 'CD4+ T cell surface alpha enolase is lower in older adults' Mechanisms of Ageing and Development, vol. 152, pp. 56–62. https://doi.org/10.1016/j.mad.2015.09.005

CD4+ T cell surface alpha enolase is lower in older adults. / Bennett, Stuart J.; Augustyniak, Edyta M.; Dunston, Christopher R.; Brown, Richard A.; Shantsila, Eduard; Lip, Gregory Y.H.; Torrão, Rita D.C.; Pararasa, Chathyan; Hussein, Ali R.; Ladouce, Romain; Friguet, Bertrand; Griffiths, Helen R.

In: Mechanisms of Ageing and Development, Vol. 152, 12.2015, p. 56–62.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CD4+ T cell surface alpha enolase is lower in older adults

AU - Bennett, Stuart J.

AU - Augustyniak, Edyta M.

AU - Dunston, Christopher R.

AU - Brown, Richard A.

AU - Shantsila, Eduard

AU - Lip, Gregory Y.H.

AU - Torrão, Rita D.C.

AU - Pararasa, Chathyan

AU - Hussein, Ali R.

AU - Ladouce, Romain

AU - Friguet, Bertrand

AU - Griffiths, Helen R.

N1 - © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Funding: MARK-AGE (EU FP7 Large-scale integrating Project HEALTH-F4-2008-200880) and COST CM1001. Supplementary data avaialbe on the jornal website.

PY - 2015/12

Y1 - 2015/12

N2 - To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4+ T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25y) and older (n=10; 50-70y) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4+ T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4+ T cells (p<0.05). In an independent age-matched case-control study, lower CD4+ T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.

AB - To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4+ T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25y) and older (n=10; 50-70y) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4+ T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4+ T cells (p<0.05). In an independent age-matched case-control study, lower CD4+ T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.

KW - enolase

KW - phenotype

KW - 2D gel electrophoresis

KW - CD4+T cell

KW - plasma membrane proteomics

UR - http://www.scopus.com/inward/record.url?scp=84944909195&partnerID=8YFLogxK

U2 - 10.1016/j.mad.2015.09.005

DO - 10.1016/j.mad.2015.09.005

M3 - Article

VL - 152

SP - 56

EP - 62

JO - Mechanisms of Ageing and Development

T2 - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

ER -

Bennett SJ, Augustyniak EM, Dunston CR, Brown RA, Shantsila E, Lip GYH et al. CD4+ T cell surface alpha enolase is lower in older adults. Mechanisms of Ageing and Development. 2015 Dec;152:56–62. https://doi.org/10.1016/j.mad.2015.09.005