Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform

Carlotta Peticone; David De Silva Thompson; Nikolay Dimov; Ben Jevans; Nick Glass; Martina Micheletti; Jonathan C Knowles; Hae-Won Kim; Justin J Cooper-White; Ivan B Wall

doi:10.1177/2041731420954712

Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform

Carlotta Peticone
, David De Silva Thompson
, Nikolay Dimov
, Ben Jevans
, Nick Glass
, Martina Micheletti
, Jonathan C Knowles
, Hae-Won Kim
, Justin J Cooper-White^*
, Ivan B Wall^*

^*Corresponding author for this work

The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Gordon St, London, WC1H 0AH, UK.
Centre for Engineering Research, University of Hertfordshire, Hatfield, Hertfordshire, UK
Developmental Neurosciences Programme, Great Ormond Street Institute of Child Health, University College London, London WC1N 3EH, United Kingdom.
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Brisbane, Australia
Division of Biomaterials and Tissue Engineering, University College London Eastman Dental Institute, London, UK; The Discoveries Centre for Regenerative and Precision Medicine, UCL Campus, London, UK; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of Korea; Institute for Tissue Regeneration Engineering, Dankook University, Cheonan, Republic of Korea
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea; UCL Eastman-Korea Dental Medicine Innovation Centre, Dankook University, Cheonan, Republic of Korea; Institute for Tissue Regeneration Engineering, Dankook University, Cheonan, Republic of Korea
Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Brisbane, Australia; School of Chemical Engineering, University of Queensland, St. Lucia, Brisbane, Australia

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

38 Downloads (Pure)

Abstract

Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol % TiO2 and either 0 mol % CoO (CoO 0%) or 2 mol % CoO (CoO 2%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.

Original language	English
Journal	Journal of Tissue Engineering
Volume	11
Early online date	24 Oct 2020
DOIs	https://doi.org/10.1177/2041731420954712
Publication status	Published - 13 Nov 2020

Bibliographical note

This article is distributed under the terms of the Creative Commons Attribution 4.0 License
(https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of
the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages
(https://us.sagepub.com/en-us/nam/open-access-at-sage).

Funding: This work was financially supported by an EPSRC Doctoral
Training Grant and Industrial Doctorate Centre in Bioprocess
Engineering Leadership (grant number: EP/G034656/1);
European Union’s Horizon 2020 research and innovation programme, under Grant agreement No 739572; National Research
Foundation (NRF), Republic of Korea (NRF2018R1A2B3003446; NRF-2018K1A4A3A01064257). It was
also funded in part by the Australian Research Council Discovery
Grants Scheme (DP140104217).

Keywords

Stem cells
microfluidics
osteogenic differentiation
phosphate glass
tissue engineering

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10.1177/2041731420954712Licence: CC BY 3.0

Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co
This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Final published version, 4.28 MBLicence: CC BY 3.0

Cite this

Peticone, C., Thompson, D. D. S., Dimov, N., Jevans, B., Glass, N., Micheletti, M., Knowles, J. C., Kim, H.-W., Cooper-White, J. J., & Wall, I. B. (2020). Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform. Journal of Tissue Engineering, 11. https://doi.org/10.1177/2041731420954712

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abstract = "Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol \% TiO2 and either 0 mol \% CoO (CoO 0\%) or 2 mol \% CoO (CoO 2\%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.",

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Characterisation of osteogenic and vascular responses of hMSCs to Ti-Co doped phosphate glass microspheres using a microfluidic perfusion platform

Abstract

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Keywords

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