Characterisation of the porcine eyeball as an in-vitro model for dry eye

Francesco Menduni, Leon N Davies, David Madrid-Costa, Antonio Fratini, James S. Wolffsohn

Research output: Contribution to journalArticle

Abstract

Purpose:
To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye.
Methods:
Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs.
Results:
Corneal steepest meridian was 7.85 ± 0.32 mm, corneal flattest meridian was 8.28 ± 0.32 mm, shortest corneal diameter was 12.69 ± 0.58 mm, longest corneal diameter was 14.88 ± 0.66 mm and central corneal ultrasonic pachymetry was 1009 ± 1μm. Anterior chamber angle was 28.83 ± 4.16°, anterior chamber depth was 1.77 ± 0.27 mm, and central corneal thickness measured using OCT was 1248 ± 144μm. Corneal endothelial cell density was 3250 ± 172 cells/mm2.
Conclusions:
Combining different clinical techniques produced a pool of reproducible data on the porcine eye anatomy, which can be used by researchers to assess the viability of using the porcine eye as an in-vitro/ex-vivo model for dry eye. Due to the similar morphology with the human eye, porcine eyeballs may represent a useful and cost effective model to individually study important key factors in the development of dry eye, such as environmental and mechanical stresses.
Original languageEnglish
Pages (from-to)13-17
JournalContact Lens and Anterior Eye
Volume41
Issue number1
Early online date3 Oct 2017
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

Swine
Anterior Chamber
Meridians
Endothelial Cells
Cell Count
Corneal Pachymetry
Mechanical Stress
In Vitro Techniques
Ultrasonics
Anatomy
Research Personnel
Costs and Cost Analysis

Bibliographical note

© 2017 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/.

Funding: European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 642760.

Keywords

  • Porcine eye
  • Dry eye
  • Confocal microscopy
  • Optical coherence tomography

Cite this

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title = "Characterisation of the porcine eyeball as an in-vitro model for dry eye",
abstract = "Purpose:To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye.Methods:Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs.Results:Corneal steepest meridian was 7.85 ± 0.32 mm, corneal flattest meridian was 8.28 ± 0.32 mm, shortest corneal diameter was 12.69 ± 0.58 mm, longest corneal diameter was 14.88 ± 0.66 mm and central corneal ultrasonic pachymetry was 1009 ± 1μm. Anterior chamber angle was 28.83 ± 4.16°, anterior chamber depth was 1.77 ± 0.27 mm, and central corneal thickness measured using OCT was 1248 ± 144μm. Corneal endothelial cell density was 3250 ± 172 cells/mm2.Conclusions:Combining different clinical techniques produced a pool of reproducible data on the porcine eye anatomy, which can be used by researchers to assess the viability of using the porcine eye as an in-vitro/ex-vivo model for dry eye. Due to the similar morphology with the human eye, porcine eyeballs may represent a useful and cost effective model to individually study important key factors in the development of dry eye, such as environmental and mechanical stresses.",
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author = "Francesco Menduni and Davies, {Leon N} and David Madrid-Costa and Antonio Fratini and Wolffsohn, {James S.}",
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Characterisation of the porcine eyeball as an in-vitro model for dry eye. / Menduni, Francesco; Davies, Leon N; Madrid-Costa, David; Fratini, Antonio; Wolffsohn, James S.

In: Contact Lens and Anterior Eye, Vol. 41, No. 1, 01.02.2018, p. 13-17.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterisation of the porcine eyeball as an in-vitro model for dry eye

AU - Menduni, Francesco

AU - Davies, Leon N

AU - Madrid-Costa, David

AU - Fratini, Antonio

AU - Wolffsohn, James S.

N1 - © 2017 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/. Funding: European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 642760.

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Purpose:To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye.Methods:Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs.Results:Corneal steepest meridian was 7.85 ± 0.32 mm, corneal flattest meridian was 8.28 ± 0.32 mm, shortest corneal diameter was 12.69 ± 0.58 mm, longest corneal diameter was 14.88 ± 0.66 mm and central corneal ultrasonic pachymetry was 1009 ± 1μm. Anterior chamber angle was 28.83 ± 4.16°, anterior chamber depth was 1.77 ± 0.27 mm, and central corneal thickness measured using OCT was 1248 ± 144μm. Corneal endothelial cell density was 3250 ± 172 cells/mm2.Conclusions:Combining different clinical techniques produced a pool of reproducible data on the porcine eye anatomy, which can be used by researchers to assess the viability of using the porcine eye as an in-vitro/ex-vivo model for dry eye. Due to the similar morphology with the human eye, porcine eyeballs may represent a useful and cost effective model to individually study important key factors in the development of dry eye, such as environmental and mechanical stresses.

AB - Purpose:To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye.Methods:Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs.Results:Corneal steepest meridian was 7.85 ± 0.32 mm, corneal flattest meridian was 8.28 ± 0.32 mm, shortest corneal diameter was 12.69 ± 0.58 mm, longest corneal diameter was 14.88 ± 0.66 mm and central corneal ultrasonic pachymetry was 1009 ± 1μm. Anterior chamber angle was 28.83 ± 4.16°, anterior chamber depth was 1.77 ± 0.27 mm, and central corneal thickness measured using OCT was 1248 ± 144μm. Corneal endothelial cell density was 3250 ± 172 cells/mm2.Conclusions:Combining different clinical techniques produced a pool of reproducible data on the porcine eye anatomy, which can be used by researchers to assess the viability of using the porcine eye as an in-vitro/ex-vivo model for dry eye. Due to the similar morphology with the human eye, porcine eyeballs may represent a useful and cost effective model to individually study important key factors in the development of dry eye, such as environmental and mechanical stresses.

KW - Porcine eye

KW - Dry eye

KW - Confocal microscopy

KW - Optical coherence tomography

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JO - Contact Lens and Anterior Eye

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SN - 1367-0484

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