Characterization at the individual cell level and in whole blood samples of shear stress preventing red blood cells aggregation

K. Lee, M. Kinnunen, A. V. Danilina, V. D. Ustinov, S. Shin*, I. Meglinski, A. V. Priezzhev

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The aggregation of red blood cells (RBC) is an intrinsic feature of blood that has a strong impact on its microcirculation. For a number of years it has been attracting a great attention in basic research and clinical studies. Here, we study a relationship between the RBC aggregation parameters measured at the individual cell level and in a whole blood sample. The home made optical tweezers were used to measure the aggregating and disaggregating forces for a pair of interacting RBCs, at the individual cell level, in order to evaluate the corresponding shear stresses. The RheoScan aggregometer was used for the measurements of critical shear stress (CSS) in whole blood samples. The correlation between CSS and the shear stress required to stop an RBC pair from aggregating was found. The shear stress required to disaggregate a pair of RBCs using the double channel optical tweezers appeared to be about 10 times higher than CSS. The correlation between shear stresses required to prevent RBCs from aggregation at the individual cell level and in whole blood samples was estimated and assessed quantitatively. The experimental approach developed has a high potential for advancing hemorheological studies.

Original languageEnglish
Pages (from-to)1021-1026
Number of pages6
JournalJournal of Biomechanics
Volume49
Issue number7
Early online date13 Feb 2016
DOIs
Publication statusPublished - 3 May 2016

Keywords

  • Critical shear stress
  • Disaggregating shear stress
  • Optical tweezers
  • Red blood cell aggregating force
  • Red blood cell aggregation

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