Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6′- dibehenate) - A novel adjuvant inducing both strong CMI and antibody responses: a novel adjuvant inducing both strong CMI and antibody responses

Jesper Davidsen, Ida Rosenkrands, Dennis Christensen, Anil K. Vangala, Daniel Kirby, Yvonne Perrie, Else M. Agger, Peter Andersen

Research output: Contribution to journalArticle

Abstract

Incorporation of the glycolipid trehalose 6,6′-dibehenate (TDB) into cationic liposomes composed of the quaternary ammonium compound dimethyldioctadecylammonium (DDA) produce an adjuvant system which induces a powerful cell-mediated immune response and a strong antibody response, desirable for a high number of disease targets. We have used differential scanning calorimetry (DSC) to investigate the effect of TDB on the gel-fluid phase transition of DDA liposomes and to demonstrate that TDB is incorporated into DDA liposome bilayers. Transmission Electron Microscopy (TEM) and cryo-TEM confirmed that liposomes were formed when a lipid film of DDA containing small amounts of TDB was hydrated in an aqueous buffer solution at physiological pH. Furthermore, time development of particle size and zeta potential of DDA liposomes incorporating TDB during storage at 4°C and 25°C, indicates that TDB effectively stabilizes the DDA liposomes. Immunization of mice with the mycobacterial fusion protein Ag85B-ESAT-6 in DDA-TDB liposomes induced a strong, specific Th1 type immune response characterized by substantial production of the interferon-γ cytokine and high levels of IgG2b isotype antibodies. The lymphocyte subset releasing the interferon-γ was identified as CD4 T cells.

Original languageEnglish
Pages (from-to)22-31
Number of pages10
JournalBBA -Biomembranes
Volume1718
Issue number1-2
DOIs
Publication statusPublished - 10 Dec 2005

Fingerprint

Cord Factors
Trehalose
Liposomes
Antibody Formation
Tuberculosis
Antibodies
Transmission Electron Microscopy
Interferons
Quaternary Ammonium Compounds
Transmission electron microscopy
Immunization
Cryoelectron Microscopy
T-cells
Lymphocytes
Glycolipids
Phase Transition
Lymphocyte Subsets
Differential Scanning Calorimetry
Zeta potential
dimethyldioctadecylammonium

Keywords

  • adjuvant
  • dimethyldioctadecylammonium
  • immunomodulator
  • liposome
  • trehalose 6,6′-dibehenate

Cite this

@article{d444860ec4a84c32af687090a8db28ec,
title = "Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6′- dibehenate) - A novel adjuvant inducing both strong CMI and antibody responses: a novel adjuvant inducing both strong CMI and antibody responses",
abstract = "Incorporation of the glycolipid trehalose 6,6′-dibehenate (TDB) into cationic liposomes composed of the quaternary ammonium compound dimethyldioctadecylammonium (DDA) produce an adjuvant system which induces a powerful cell-mediated immune response and a strong antibody response, desirable for a high number of disease targets. We have used differential scanning calorimetry (DSC) to investigate the effect of TDB on the gel-fluid phase transition of DDA liposomes and to demonstrate that TDB is incorporated into DDA liposome bilayers. Transmission Electron Microscopy (TEM) and cryo-TEM confirmed that liposomes were formed when a lipid film of DDA containing small amounts of TDB was hydrated in an aqueous buffer solution at physiological pH. Furthermore, time development of particle size and zeta potential of DDA liposomes incorporating TDB during storage at 4°C and 25°C, indicates that TDB effectively stabilizes the DDA liposomes. Immunization of mice with the mycobacterial fusion protein Ag85B-ESAT-6 in DDA-TDB liposomes induced a strong, specific Th1 type immune response characterized by substantial production of the interferon-γ cytokine and high levels of IgG2b isotype antibodies. The lymphocyte subset releasing the interferon-γ was identified as CD4 T cells.",
keywords = "adjuvant, dimethyldioctadecylammonium, immunomodulator, liposome, trehalose 6,6′-dibehenate",
author = "Jesper Davidsen and Ida Rosenkrands and Dennis Christensen and Vangala, {Anil K.} and Daniel Kirby and Yvonne Perrie and Agger, {Else M.} and Peter Andersen",
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Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6′- dibehenate) - A novel adjuvant inducing both strong CMI and antibody responses : a novel adjuvant inducing both strong CMI and antibody responses. / Davidsen, Jesper; Rosenkrands, Ida; Christensen, Dennis; Vangala, Anil K.; Kirby, Daniel; Perrie, Yvonne; Agger, Else M.; Andersen, Peter.

In: BBA -Biomembranes, Vol. 1718, No. 1-2, 10.12.2005, p. 22-31.

Research output: Contribution to journalArticle

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T1 - Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6′- dibehenate) - A novel adjuvant inducing both strong CMI and antibody responses

T2 - a novel adjuvant inducing both strong CMI and antibody responses

AU - Davidsen, Jesper

AU - Rosenkrands, Ida

AU - Christensen, Dennis

AU - Vangala, Anil K.

AU - Kirby, Daniel

AU - Perrie, Yvonne

AU - Agger, Else M.

AU - Andersen, Peter

PY - 2005/12/10

Y1 - 2005/12/10

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KW - immunomodulator

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KW - trehalose 6,6′-dibehenate

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