Characterization of FITC-conjugated lectin binding to Candida albicans

Stephen N. Smith, Richard A. Armstrong, Roger A. Bird, Rita Chohan, Nick A. Hartell, David A. Poyner

Research output: Contribution to journalArticle

Abstract

Avidity of yeast and hyphal forms of Candida albicans for FITC-conjugated lectins was determined by flow cytometry and digital microscopy. Yeast phase cells bound Con A, a lectin with marked affinity for mannose, irrespective of growth phase, yet demonstrated little avidity for WGA and SBA. Yeast phase cell avidity for mannose-specific lectins was characterized through determination of FITC-conjugated Con A, LcH, PSA and GNA binding and subsequent calculation of Bmax, EC50 and Hn values. Such an approach, through comparison among FITC-conjugated lectins of differing specific activities, furnishes further insight into exposed outer cell wall mannose moieties. The rank order of lectin affinity as defined by EC50 values was GNA > Con A > LcH > PSA. Values for Hn suggest that lectins predominantly bind to a single receptor class, the relative abundance of which as defined by Bmax values was PSA > GNA > Con A > LcH. Hyphal surfaces in common with yeast phase cells demonstrated marked avidity for FITC-Con A, however, fluorescence of Candida morphological forms differed significantly, indicative of varying outer cell wall mannose exposure.

Original languageEnglish
Pages (from-to)422-431
Number of pages10
JournalMycologia
Volume93
Issue number3
Publication statusPublished - 9 Oct 2002

Fingerprint

Fluorescein-5-isothiocyanate
Candida albicans
Lectins
lectins
yeast
concanavalin A
mannose
Mannose
Yeasts
yeasts
Cell Wall
Mannose-Binding Lectins
flow cytometry
cell walls
microscopy
relative abundance
fluorescence
Candida
cells
Microscopy

Keywords

  • avidity
  • flourescence
  • hyphal
  • mannose
  • yeast

Cite this

Smith, S. N., Armstrong, R. A., Bird, R. A., Chohan, R., Hartell, N. A., & Poyner, D. A. (2002). Characterization of FITC-conjugated lectin binding to Candida albicans. Mycologia, 93(3), 422-431.
Smith, Stephen N. ; Armstrong, Richard A. ; Bird, Roger A. ; Chohan, Rita ; Hartell, Nick A. ; Poyner, David A. / Characterization of FITC-conjugated lectin binding to Candida albicans. In: Mycologia. 2002 ; Vol. 93, No. 3. pp. 422-431.
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Smith, SN, Armstrong, RA, Bird, RA, Chohan, R, Hartell, NA & Poyner, DA 2002, 'Characterization of FITC-conjugated lectin binding to Candida albicans', Mycologia, vol. 93, no. 3, pp. 422-431.

Characterization of FITC-conjugated lectin binding to Candida albicans. / Smith, Stephen N.; Armstrong, Richard A.; Bird, Roger A.; Chohan, Rita; Hartell, Nick A.; Poyner, David A.

In: Mycologia, Vol. 93, No. 3, 09.10.2002, p. 422-431.

Research output: Contribution to journalArticle

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Smith SN, Armstrong RA, Bird RA, Chohan R, Hartell NA, Poyner DA. Characterization of FITC-conjugated lectin binding to Candida albicans. Mycologia. 2002 Oct 9;93(3):422-431.