Circular polarization biomicroscopy: a method for determining human corneal stromal lamellar organization in vivo

Research output: Contribution to journalArticle

Abstract

The theory of polarization biomicroscopy is explored using Stokes vectors and Mueller matrices. It is established that circular polarization can be used to simultaneously detect birefringent elements at any orientation unlike orientation-sensitive techniques using linear polarized light alone. A method of biomicroscopy using circular polarized light is described and tested in a physical model. The method is then used to investigate the lamellar structure of human corneas in vivo in pairs of eyes of 38 subjects. An approximate confocal elliptic/hyperbolic distribution of stromal fibrils, presumed to be collagen, is clearly identified within central and intermediate areas of the cornea. All subjects tested demonstrate approximate mirror symmetry between pairs of eyes typically with a preferred orientation of central fibrils at approximately 15 degrees to the horizontal in a superotemporal-inferonasal direction.

Original languageEnglish
Pages (from-to)256-264
Number of pages9
JournalOphthalmic and Physiological Optics
Volume27
Issue number3
DOIs
Publication statusPublished - May 2007

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Cornea
Light
Collagen
Direction compound

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Collagen/ultrastructure
  • Cornea/physiology
  • Corneal Stroma/ultrastructure
  • Female
  • Humans
  • Male
  • Microscopy, Polarization/methods
  • Middle Aged
  • Models, Biological
  • Ocular Physiological Phenomena

Cite this

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title = "Circular polarization biomicroscopy: a method for determining human corneal stromal lamellar organization in vivo",
abstract = "The theory of polarization biomicroscopy is explored using Stokes vectors and Mueller matrices. It is established that circular polarization can be used to simultaneously detect birefringent elements at any orientation unlike orientation-sensitive techniques using linear polarized light alone. A method of biomicroscopy using circular polarized light is described and tested in a physical model. The method is then used to investigate the lamellar structure of human corneas in vivo in pairs of eyes of 38 subjects. An approximate confocal elliptic/hyperbolic distribution of stromal fibrils, presumed to be collagen, is clearly identified within central and intermediate areas of the cornea. All subjects tested demonstrate approximate mirror symmetry between pairs of eyes typically with a preferred orientation of central fibrils at approximately 15 degrees to the horizontal in a superotemporal-inferonasal direction.",
keywords = "Adult, Aged, Aged, 80 and over, Collagen/ultrastructure, Cornea/physiology, Corneal Stroma/ultrastructure, Female, Humans, Male, Microscopy, Polarization/methods, Middle Aged, Models, Biological, Ocular Physiological Phenomena",
author = "Misson, {Gary P}",
year = "2007",
month = "5",
doi = "10.1111/j.1475-1313.2007.00482.x",
language = "English",
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TY - JOUR

T1 - Circular polarization biomicroscopy

T2 - a method for determining human corneal stromal lamellar organization in vivo

AU - Misson, Gary P

PY - 2007/5

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AB - The theory of polarization biomicroscopy is explored using Stokes vectors and Mueller matrices. It is established that circular polarization can be used to simultaneously detect birefringent elements at any orientation unlike orientation-sensitive techniques using linear polarized light alone. A method of biomicroscopy using circular polarized light is described and tested in a physical model. The method is then used to investigate the lamellar structure of human corneas in vivo in pairs of eyes of 38 subjects. An approximate confocal elliptic/hyperbolic distribution of stromal fibrils, presumed to be collagen, is clearly identified within central and intermediate areas of the cornea. All subjects tested demonstrate approximate mirror symmetry between pairs of eyes typically with a preferred orientation of central fibrils at approximately 15 degrees to the horizontal in a superotemporal-inferonasal direction.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Collagen/ultrastructure

KW - Cornea/physiology

KW - Corneal Stroma/ultrastructure

KW - Female

KW - Humans

KW - Male

KW - Microscopy, Polarization/methods

KW - Middle Aged

KW - Models, Biological

KW - Ocular Physiological Phenomena

UR - https://onlinelibrary.wiley.com/doi/full/10.1111/j.1475-1313.2007.00482.x

U2 - 10.1111/j.1475-1313.2007.00482.x

DO - 10.1111/j.1475-1313.2007.00482.x

M3 - Article

C2 - 17470238

VL - 27

SP - 256

EP - 264

JO - Ophthalmic and Physiological Optics

JF - Ophthalmic and Physiological Optics

SN - 0275-5408

IS - 3

ER -