Comparison and characterization of different polyester nano/micro fibres for use in tissue engineering applications

Petr Mikes, Jana Horakova, Ales Saman, Lucie Vejsadova, Paul Topham, Winita Punyodom, Manita Dumklang, Vera Jencova

Research output: Contribution to journalArticle

Abstract

The study focuses on a comparison of the electrospinning of various polylactide and polycaprolactone (PLCL) copolymers and poly-L-lactide (PLLA) and polycaprolactone (PCL) homopolymers. The chemical characterisation, electrospinnability, fibrous morphology, degradation rate and interactions with fibroblasts were assessed with respect to copolymers and homopolymers with both lower (around 50,000) and higher (around 95,000) molecular weights. The research investigated commercially available as well as synthesised copolymers. The results revealed that the electrospinnability of polymeric solutions depends on both the molecular weight and the PLA/PCL ratio in the final copolymer. It was determined that PLCL copolymers with a higher content of PCL (≥20%) were not spinnable via the electrospinning process. With the exception of PCL, the resulting fibrous materials were found to be homogeneous and with fibre diameters of slightly more than 1 µm with respect to both the tested molecular weights. The degradation rate was tested under simulation conditions via the utilisation of the lipase and Proteinase K enzymes. The degree of degradation was found to depend on the molecular weight, the crystallinity of the polymer and the specificity of the enzyme applied. While lipase was responsible for the degradation of the PCL polymer, it exerted a minor impact on the PLLA and the copolymers. Proteinase K degraded all the tested polymers with a higher specificity towards PLLA and the PLCL copolymers. All the tested polymers were affected by the surface erosion degradation process via fibrous morphology changes and mass loss with no accompanying shift in the molar mass. The electrospun PLLA materials supported both fibroblast adhesion and proliferation. All the tested materials were determined to be cytocompatible with 3T3 mouse fibroblasts.

Original languageEnglish
JournalJournal of Industrial Textiles
Early online date8 May 2019
DOIs
Publication statusE-pub ahead of print - 8 May 2019

Fingerprint

Polycaprolactone
Polyesters
Tissue engineering
Copolymers
Fibers
Polymers
Degradation
Fibroblasts
Molecular weight
Endopeptidase K
Lipases
Electrospinning
Homopolymerization
Lipase
Enzymes
polycaprolactone
Molar mass
Erosion
Adhesion
poly(lactide)

Keywords

  • Polyesters
  • biodegradability
  • copolymers
  • cytocompatibility
  • electrospinning

Cite this

Mikes, P., Horakova, J., Saman, A., Vejsadova, L., Topham, P., Punyodom, W., ... Jencova, V. (2019). Comparison and characterization of different polyester nano/micro fibres for use in tissue engineering applications. Journal of Industrial Textiles. https://doi.org/10.1177/1528083719848155
Mikes, Petr ; Horakova, Jana ; Saman, Ales ; Vejsadova, Lucie ; Topham, Paul ; Punyodom, Winita ; Dumklang, Manita ; Jencova, Vera. / Comparison and characterization of different polyester nano/micro fibres for use in tissue engineering applications. In: Journal of Industrial Textiles. 2019.
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abstract = "The study focuses on a comparison of the electrospinning of various polylactide and polycaprolactone (PLCL) copolymers and poly-L-lactide (PLLA) and polycaprolactone (PCL) homopolymers. The chemical characterisation, electrospinnability, fibrous morphology, degradation rate and interactions with fibroblasts were assessed with respect to copolymers and homopolymers with both lower (around 50,000) and higher (around 95,000) molecular weights. The research investigated commercially available as well as synthesised copolymers. The results revealed that the electrospinnability of polymeric solutions depends on both the molecular weight and the PLA/PCL ratio in the final copolymer. It was determined that PLCL copolymers with a higher content of PCL (≥20{\%}) were not spinnable via the electrospinning process. With the exception of PCL, the resulting fibrous materials were found to be homogeneous and with fibre diameters of slightly more than 1 µm with respect to both the tested molecular weights. The degradation rate was tested under simulation conditions via the utilisation of the lipase and Proteinase K enzymes. The degree of degradation was found to depend on the molecular weight, the crystallinity of the polymer and the specificity of the enzyme applied. While lipase was responsible for the degradation of the PCL polymer, it exerted a minor impact on the PLLA and the copolymers. Proteinase K degraded all the tested polymers with a higher specificity towards PLLA and the PLCL copolymers. All the tested polymers were affected by the surface erosion degradation process via fibrous morphology changes and mass loss with no accompanying shift in the molar mass. The electrospun PLLA materials supported both fibroblast adhesion and proliferation. All the tested materials were determined to be cytocompatible with 3T3 mouse fibroblasts.",
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Mikes, P, Horakova, J, Saman, A, Vejsadova, L, Topham, P, Punyodom, W, Dumklang, M & Jencova, V 2019, 'Comparison and characterization of different polyester nano/micro fibres for use in tissue engineering applications', Journal of Industrial Textiles. https://doi.org/10.1177/1528083719848155

Comparison and characterization of different polyester nano/micro fibres for use in tissue engineering applications. / Mikes, Petr; Horakova, Jana; Saman, Ales; Vejsadova, Lucie; Topham, Paul; Punyodom, Winita; Dumklang, Manita; Jencova, Vera.

In: Journal of Industrial Textiles, 08.05.2019.

Research output: Contribution to journalArticle

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T1 - Comparison and characterization of different polyester nano/micro fibres for use in tissue engineering applications

AU - Mikes, Petr

AU - Horakova, Jana

AU - Saman, Ales

AU - Vejsadova, Lucie

AU - Topham, Paul

AU - Punyodom, Winita

AU - Dumklang, Manita

AU - Jencova, Vera

PY - 2019/5/8

Y1 - 2019/5/8

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AB - The study focuses on a comparison of the electrospinning of various polylactide and polycaprolactone (PLCL) copolymers and poly-L-lactide (PLLA) and polycaprolactone (PCL) homopolymers. The chemical characterisation, electrospinnability, fibrous morphology, degradation rate and interactions with fibroblasts were assessed with respect to copolymers and homopolymers with both lower (around 50,000) and higher (around 95,000) molecular weights. The research investigated commercially available as well as synthesised copolymers. The results revealed that the electrospinnability of polymeric solutions depends on both the molecular weight and the PLA/PCL ratio in the final copolymer. It was determined that PLCL copolymers with a higher content of PCL (≥20%) were not spinnable via the electrospinning process. With the exception of PCL, the resulting fibrous materials were found to be homogeneous and with fibre diameters of slightly more than 1 µm with respect to both the tested molecular weights. The degradation rate was tested under simulation conditions via the utilisation of the lipase and Proteinase K enzymes. The degree of degradation was found to depend on the molecular weight, the crystallinity of the polymer and the specificity of the enzyme applied. While lipase was responsible for the degradation of the PCL polymer, it exerted a minor impact on the PLLA and the copolymers. Proteinase K degraded all the tested polymers with a higher specificity towards PLLA and the PLCL copolymers. All the tested polymers were affected by the surface erosion degradation process via fibrous morphology changes and mass loss with no accompanying shift in the molar mass. The electrospun PLLA materials supported both fibroblast adhesion and proliferation. All the tested materials were determined to be cytocompatible with 3T3 mouse fibroblasts.

KW - Polyesters

KW - biodegradability

KW - copolymers

KW - cytocompatibility

KW - electrospinning

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