The paper describes the experiment at pancreas ischemia model carried out by combining the methods of fluorescence spectroscopy and laser speckle contrast imaging in laboratory mice. The measurement was performed before and after ischemia to evaluate the relationship between the changes of blood flow and metabolic activity. The results showed high positive correlation between fluorescence intensity at excitation wavelength of 365 nm and speckle contrast. At the same time, 450 nm fluorescence intensity and speckle contrast showed high negative correlation as well as two kinds of fluorescence maxima. The proposed approach seems promising for further studies and future clinical testing.
|Proceedings of SPIE - The International Society for Optical Engineering
|Published - 21 Apr 2019
|Biomedical Imaging and Sensing Conference 2019, BISC 2019 - Yokohama, Japan
Duration: 22 Apr 2019 → 26 Apr 2019
- Fluorescence spectroscopy
- Speckle contrast