Abstract
The preparation and characterisation of collagen:PCL composites for manufacture of tissue engineered skin substitutes and models are reported. Films having collagen:PCL (w/w) ratios of 1:4, 1:8 and 1:20 were prepared by impregnation of lyophilised collagen mats by PCL solutions followed by solvent evaporation. In vitro assays of collagen release and residual collagen content revealed an expected inverse relationship between the collagen release rate and the content of synthetic polymer in the composite that may be exploited for controlled presentation and release of biopharmaceuticals such as growth factors. DSC analysis revealed the characteristic melting point of PCL at around 60°C and a tendency for the collagen component, at high loading, to impede crystallinity development within the PCL phase. The preparation of fibroblast/composite constructs was investigated using cell culture as a first stage in mimicking the dermal/epidermal structure of skin. Fibroblasts were found to attach and proliferate on all the composites investigated reaching a maximum of 2×105/cm2 on 1:20 collagen:PCL materials at day 8 with cell numbers declining thereafter. Keratinocyte growth rates were similar on all types of collagen:PCL materials investigated reaching a maximum of 6.6×104/cm2 at day 6. The results revealed that composite films of collagen and PCL are favourable substrates for growth of fibroblasts and keratinocytes and may find utility for skin repair. © 2003 Elsevier Ltd. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 4263-4271 |
| Number of pages | 9 |
| Journal | Biomaterials |
| Volume | 25 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - Aug 2004 |
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Keywords
- cell culture
- collagen
- composite
- fibroblast
- keratinocyte
- polycaprolactone
- skin substitutes
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Composite cell support membranes based on collagen and polycaprolactone for tissue engineering of skin. / Dai, Niann-Tzyy; Williamson, Matthew R.; Khammo, N.; Adams, Eric F.; Coombes, Allan G.A.
In: Biomaterials, Vol. 25, No. 18, 08.2004, p. 4263-4271.Research output: Contribution to journal › Article
TY - JOUR
T1 - Composite cell support membranes based on collagen and polycaprolactone for tissue engineering of skin
AU - Dai, Niann-Tzyy
AU - Williamson, Matthew R.
AU - Khammo, N.
AU - Adams, Eric F.
AU - Coombes, Allan G.A.
PY - 2004/8
Y1 - 2004/8
N2 - The preparation and characterisation of collagen:PCL composites for manufacture of tissue engineered skin substitutes and models are reported. Films having collagen:PCL (w/w) ratios of 1:4, 1:8 and 1:20 were prepared by impregnation of lyophilised collagen mats by PCL solutions followed by solvent evaporation. In vitro assays of collagen release and residual collagen content revealed an expected inverse relationship between the collagen release rate and the content of synthetic polymer in the composite that may be exploited for controlled presentation and release of biopharmaceuticals such as growth factors. DSC analysis revealed the characteristic melting point of PCL at around 60°C and a tendency for the collagen component, at high loading, to impede crystallinity development within the PCL phase. The preparation of fibroblast/composite constructs was investigated using cell culture as a first stage in mimicking the dermal/epidermal structure of skin. Fibroblasts were found to attach and proliferate on all the composites investigated reaching a maximum of 2×105/cm2 on 1:20 collagen:PCL materials at day 8 with cell numbers declining thereafter. Keratinocyte growth rates were similar on all types of collagen:PCL materials investigated reaching a maximum of 6.6×104/cm2 at day 6. The results revealed that composite films of collagen and PCL are favourable substrates for growth of fibroblasts and keratinocytes and may find utility for skin repair. © 2003 Elsevier Ltd. All rights reserved.
AB - The preparation and characterisation of collagen:PCL composites for manufacture of tissue engineered skin substitutes and models are reported. Films having collagen:PCL (w/w) ratios of 1:4, 1:8 and 1:20 were prepared by impregnation of lyophilised collagen mats by PCL solutions followed by solvent evaporation. In vitro assays of collagen release and residual collagen content revealed an expected inverse relationship between the collagen release rate and the content of synthetic polymer in the composite that may be exploited for controlled presentation and release of biopharmaceuticals such as growth factors. DSC analysis revealed the characteristic melting point of PCL at around 60°C and a tendency for the collagen component, at high loading, to impede crystallinity development within the PCL phase. The preparation of fibroblast/composite constructs was investigated using cell culture as a first stage in mimicking the dermal/epidermal structure of skin. Fibroblasts were found to attach and proliferate on all the composites investigated reaching a maximum of 2×105/cm2 on 1:20 collagen:PCL materials at day 8 with cell numbers declining thereafter. Keratinocyte growth rates were similar on all types of collagen:PCL materials investigated reaching a maximum of 6.6×104/cm2 at day 6. The results revealed that composite films of collagen and PCL are favourable substrates for growth of fibroblasts and keratinocytes and may find utility for skin repair. © 2003 Elsevier Ltd. All rights reserved.
KW - cell culture
KW - collagen
KW - composite
KW - fibroblast
KW - keratinocyte
KW - polycaprolactone
KW - skin substitutes
UR - http://www.scopus.com/inward/record.url?scp=1642406852&partnerID=8YFLogxK
UR - https://www.sciencedirect.com/science/article/pii/S014296120301086X?via%3Dihub
U2 - 10.1016/j.biomaterials.2003.11.022
DO - 10.1016/j.biomaterials.2003.11.022
M3 - Article
C2 - 15046916
VL - 25
SP - 4263
EP - 4271
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 18
ER -