Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome

Xiaoping Yang; Yishai Levin; Hassan Rahmoune; Dan Ma; Stephanie Schöffmann; Yagnesh Umrania; Paul C. Guest; Sabine Bahn

doi:10.1002/pmic.201000525

Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome

Xiaoping Yang
, Yishai Levin^*
, Hassan Rahmoune
, Dan Ma
, Stephanie Schöffmann
, Yagnesh Umrania
, Paul C. Guest
, Sabine Bahn

^*Corresponding author for this work

Aston Medical School

University of Cambridge
Weizmann Institute of Science
Erasmus University Medical Center

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator^® system, we identified 1340 unique proteins. Functional classification showed that most of these were associated with synaptic function and comprised a high proportion of phosphorylated proteins and analytically challenging classes of membrane proteins such as ion channel receptor subunits.

Original language	English
Pages (from-to)	501-505
Number of pages	5
Journal	Proteomics
Volume	11
Issue number	3
DOIs	https://doi.org/10.1002/pmic.201000525
Publication status	Published - Feb 2011

Keywords

2-D liquid chromatography
Animal proteomics
Hippocampus
Mass spectrometry

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10.1002/pmic.201000525

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AU - Schöffmann, Stephanie

AU - Umrania, Yagnesh

AU - Guest, Paul C.

AU - Bahn, Sabine

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KW - 2-D liquid chromatography

KW - Animal proteomics

KW - Hippocampus

KW - Mass spectrometry

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Comprehensive two-dimensional liquid chromatography mass spectrometric profiling of the rat hippocampal proteome

Abstract

Keywords

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