Cooperation between osteoblastic cells and endothelial cells enhances their phenotypic responses and improves osteoblast function

Tuvd Dariima, Guang-Zhen Jin, Eun-Jung Lee, Ivan B Wall, Hae-Won Kim

Research output: Contribution to journalArticle

Abstract

Osteogenesis requires close co-operation with angiogenesis to create vascularized bone tissue. In this study, an indirect co-culture model using osteoblasts (OBs), primary endothelial cells (ECs) and Matrigel interlayer was established to understand the impact of each cell type on the other. ECs synergistically enhanced osteoblastic gene expression by OBs, while OBs were capable of supporting tubule-like structures formed by ECs on Matrigel, enhancing mean tubule length from 146.5 ± 23.5 μm in ECs alone to 192 ± 28.6 μm in co-culture (p < 0.05). Similar improvements were noted in terms of tubule number. An applicability study of the co-culture model to bone tissue engineering, performed on a biopolymer fibrous membrane, showed substantially enhanced deposition of calcified nodules. These results demonstrate the efficacy of co-culture with ECs to improve osteogenesis for bone tissue engineering.

Original languageEnglish
Pages (from-to)1135-1143
Number of pages9
JournalBiotechnology Letters
Volume35
Issue number7
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Osteoblasts
Endothelial cells
Coculture Techniques
Endothelial Cells
Cell culture
Bone
Tissue Engineering
Tissue engineering
Osteogenesis
Bone and Bones
Fibrous membranes
Biopolymers
Gene expression
Tissue
Gene Expression
Membranes
matrigel

Keywords

  • Bone Development
  • Coculture Techniques/methods
  • Collagen
  • Drug Combinations
  • Endothelial Cells/physiology
  • Laminin
  • Osteoblasts/physiology
  • Proteoglycans
  • Tissue Engineering/methods

Cite this

Dariima, Tuvd ; Jin, Guang-Zhen ; Lee, Eun-Jung ; Wall, Ivan B ; Kim, Hae-Won. / Cooperation between osteoblastic cells and endothelial cells enhances their phenotypic responses and improves osteoblast function. In: Biotechnology Letters. 2013 ; Vol. 35, No. 7. pp. 1135-1143.
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abstract = "Osteogenesis requires close co-operation with angiogenesis to create vascularized bone tissue. In this study, an indirect co-culture model using osteoblasts (OBs), primary endothelial cells (ECs) and Matrigel interlayer was established to understand the impact of each cell type on the other. ECs synergistically enhanced osteoblastic gene expression by OBs, while OBs were capable of supporting tubule-like structures formed by ECs on Matrigel, enhancing mean tubule length from 146.5 ± 23.5 μm in ECs alone to 192 ± 28.6 μm in co-culture (p < 0.05). Similar improvements were noted in terms of tubule number. An applicability study of the co-culture model to bone tissue engineering, performed on a biopolymer fibrous membrane, showed substantially enhanced deposition of calcified nodules. These results demonstrate the efficacy of co-culture with ECs to improve osteogenesis for bone tissue engineering.",
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Cooperation between osteoblastic cells and endothelial cells enhances their phenotypic responses and improves osteoblast function. / Dariima, Tuvd; Jin, Guang-Zhen; Lee, Eun-Jung; Wall, Ivan B; Kim, Hae-Won.

In: Biotechnology Letters, Vol. 35, No. 7, 07.2013, p. 1135-1143.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cooperation between osteoblastic cells and endothelial cells enhances their phenotypic responses and improves osteoblast function

AU - Dariima, Tuvd

AU - Jin, Guang-Zhen

AU - Lee, Eun-Jung

AU - Wall, Ivan B

AU - Kim, Hae-Won

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KW - Coculture Techniques/methods

KW - Collagen

KW - Drug Combinations

KW - Endothelial Cells/physiology

KW - Laminin

KW - Osteoblasts/physiology

KW - Proteoglycans

KW - Tissue Engineering/methods

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