Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus

Emily J. Baker; Gemma Allcott; Antonia Molloy; Jonathan A. G. Cox

doi:10.1038/s44259-024-00054-3

Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus

Emily J. Baker
, Gemma Allcott
, Antonia Molloy
, Jonathan A. G. Cox^*

^*Corresponding author for this work

Aston University

Research output: Contribution to journal › Article › peer-review

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Abstract

Mycobacterium abscessus complex (MABSC) comprises a group of environmental microorganisms, which are a concerning cause of opportunistic respiratory infections in patients with cystic fibrosis or bronchiectasis. Only 45.6% of MABSC treatments are successful, and therefore this is a need to discover new antimicrobials that can treat these pathogens. However, the transferability of outcomes to the clinic is flawed by an inability to accurately represent the lung environment within the laboratory. Herein, we apply two preestablished formulations of sputum media (ACFS and SCFM1) to MABSC antibiotic susceptibility testing. Using conventional broth microdilution, we have observed strain and antibiotic dependent alterations in antimicrobial sensitivity in each sputum media compared standard laboratory media (7H9), with an overall reduction in susceptibility within the physiologically relevant conditions. We provide a timely contribution to the field of M. abscessus antibiotic discovery by emphasising the need for improved physiological relevance.

Original language	English
Article number	34
Number of pages	8
Journal	npj Antimicrobials and Resistance
Volume	2
Issue number	1
Early online date	5 Nov 2024
DOIs	https://doi.org/10.1038/s44259-024-00054-3
Publication status	Published - 5 Nov 2024

Bibliographical note

Copyright © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/

Funding

This research was funded by the Engineering and Physical Sciences Research Council (EPSRC) and Science Foundation Ireland (SFI) Centre for Doctoral Training in Engineered Tissues for Discovery, Industry and Medicine (EP/S02347X/1). EJB and AM are supported by a PhD Studentship funded by LifETIME CDT (EPSRC and SFI). GA is supported by a co-funded PhD studentship between Aston University and Comvita, NZ. The funders played no role in study design, data collection, analysis and interpretation of data, or the writing of the manuscript.

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10.1038/s44259-024-00054-3Licence: CC BY 4.0

EJ Baker et al_Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus
Copyright © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/
Final published version, 897 KBLicence: CC BY 4.0

Cite this

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abstract = "Mycobacterium abscessus complex (MABSC) comprises a group of environmental microorganisms, which are a concerning cause of opportunistic respiratory infections in patients with cystic fibrosis or bronchiectasis. Only 45.6\% of MABSC treatments are successful, and therefore this is a need to discover new antimicrobials that can treat these pathogens. However, the transferability of outcomes to the clinic is flawed by an inability to accurately represent the lung environment within the laboratory. Herein, we apply two preestablished formulations of sputum media (ACFS and SCFM1) to MABSC antibiotic susceptibility testing. Using conventional broth microdilution, we have observed strain and antibiotic dependent alterations in antimicrobial sensitivity in each sputum media compared standard laboratory media (7H9), with an overall reduction in susceptibility within the physiologically relevant conditions. We provide a timely contribution to the field of M. abscessus antibiotic discovery by emphasising the need for improved physiological relevance.",

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Cystic fibrosis sputum media induces an overall loss of antibiotic susceptibility in Mycobacterium abscessus

Abstract

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