Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver

Luis M. Veggi, Luciana Pretto, Elena J. Ochoa, Viviana A. Catania, Marcelo G. Luquita, Diego R. Taborda, Enrique J. Sánchez Pozzi, Shin-ichi Ikushiro, Michael D. Coleman, Marcelo G. Roma, Aldo D. Mottino

Research output: Contribution to journalArticle

Abstract

Dapsone (DDS) is currently used in the treatment of leprosy, malaria and in infections with Pneumocystis jirovecii and Toxoplasma gondii in AIDS patients. Adverse effects of DDS involve methemoglobinemia and hemolysis and, to a lower extent, liver damage, though the mechanism is poorly characterized. We evaluated the effect of DDS administration to male and female rats (30 mg/kg body wt, twice a day, for 4 days) on liver oxidative stress through assessment of biliary output and liver content of reduced (GSH) and oxidized (GSSG) glutathione, lipid peroxidation, and expression/activities of the main antioxidant enzymes glutathione peroxidase, superoxide dismutase, catalase and glutathione S-transferase. The influence of DDS treatment on express ion/activity of the main DDS phase-II- metabolizing system, UDP-glucuronosyltransferase (UGT), was additionally evaluated. The involvement of dapsone hydroxylamine (DDS-NHOH) generation in these processes was estimated by comparing the data in male and female rats since N-hydroxylation of DDS mainly occurs in males. Our studies revealed an increase in the GSSG/GSH biliary output ratio, a sensitive indicator of oxidative stress, and in lipid peroxiclation, in male but not in female rats treated with DDS. The activity of all antioxidant enzymes was significantly impaired by DDS treatment also in male rats, whereas UGT activity was not affected in any sex. Taken together, the evidence indicates that DDS induces oxidative stress in rat liver and that N-hydroxylation of DDS was the likely mediator. Impairment in the activity of enzymatic antioxidant systems, also associated with DDS-NHOH formation, constituted a key aggravating factor.
LanguageEnglish
Pages155-163
Number of pages9
JournalLife Sciences
Volume83
Issue number5-6
Early online date18 Jun 2008
DOIs
Publication statusPublished - 1 Aug 2008

Fingerprint

Dapsone
Oxidative stress
Liver
Rats
Oxidative Stress
Antioxidants
Glutathione Disulfide
Glucuronosyltransferase
Hydroxylation
Methemoglobinemia
Lipids
Pneumocystis carinii
Toxoplasma
Leprosy
Enzymes
Glutathione Peroxidase
Hemolysis
Glutathione Transferase
Catalase
Lipid Peroxidation

Keywords

  • dapsone
  • dapsone hydroxylamine
  • oxidative stress
  • lipid peroxidation
  • antioxidant enzymes
  • glutathione S-transferase
  • UDP-glucuronosyltransferase

Cite this

Veggi, L. M., Pretto, L., Ochoa, E. J., Catania, V. A., Luquita, M. G., Taborda, D. R., ... Mottino, A. D. (2008). Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver. Life Sciences, 83(5-6), 155-163. https://doi.org/10.1016/j.lfs.2008.05.016
Veggi, Luis M. ; Pretto, Luciana ; Ochoa, Elena J. ; Catania, Viviana A. ; Luquita, Marcelo G. ; Taborda, Diego R. ; Sánchez Pozzi, Enrique J. ; Ikushiro, Shin-ichi ; Coleman, Michael D. ; Roma, Marcelo G. ; Mottino, Aldo D. / Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver. In: Life Sciences. 2008 ; Vol. 83, No. 5-6. pp. 155-163.
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Veggi, LM, Pretto, L, Ochoa, EJ, Catania, VA, Luquita, MG, Taborda, DR, Sánchez Pozzi, EJ, Ikushiro, S, Coleman, MD, Roma, MG & Mottino, AD 2008, 'Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver' Life Sciences, vol. 83, no. 5-6, pp. 155-163. https://doi.org/10.1016/j.lfs.2008.05.016

Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver. / Veggi, Luis M.; Pretto, Luciana; Ochoa, Elena J.; Catania, Viviana A.; Luquita, Marcelo G.; Taborda, Diego R.; Sánchez Pozzi, Enrique J.; Ikushiro, Shin-ichi; Coleman, Michael D.; Roma, Marcelo G.; Mottino, Aldo D.

In: Life Sciences, Vol. 83, No. 5-6, 01.08.2008, p. 155-163.

Research output: Contribution to journalArticle

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T1 - Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver

AU - Veggi, Luis M.

AU - Pretto, Luciana

AU - Ochoa, Elena J.

AU - Catania, Viviana A.

AU - Luquita, Marcelo G.

AU - Taborda, Diego R.

AU - Sánchez Pozzi, Enrique J.

AU - Ikushiro, Shin-ichi

AU - Coleman, Michael D.

AU - Roma, Marcelo G.

AU - Mottino, Aldo D.

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Veggi LM, Pretto L, Ochoa EJ, Catania VA, Luquita MG, Taborda DR et al. Dapsone induces oxidative stress and impairs antioxidant defenses in rat liver. Life Sciences. 2008 Aug 1;83(5-6):155-163. https://doi.org/10.1016/j.lfs.2008.05.016