Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics

Khushboo Borah; Olivia J. Rickman; Nikol Voutsina; Emma L. Baple; Irundika HK Dias; Andrew H. Crosby; Helen R. Griffiths

doi:10.1016/j.dib.2020.106382

Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics

Khushboo Borah, Olivia J. Rickman, Nikol Voutsina, Emma L. Baple, Irundika HK Dias, Andrew H. Crosby, Helen R. Griffiths

Research output: Contribution to journal › Article › peer-review

Abstract

The raw datasets of oxysterol quantifications from whole cell and mitochondrial fractions of THP-1 monocytes and macrophages, neuronal-like SH-SH5Y cells and human peripheral blood mononuclear cells are presented. Oxysterols were quantified using a new liquid chromatography-mass spectrometry (LC-MS) and multiple reaction monitoring analysis published in the article “A quantitative LC-MS/MS method for analysis of mitochondrial-specific oxysterol metabolism” in Redox Biology [1]. This method showed improved extraction efficiency and recovery of mono and dihydroxycholesterols from cellular matrix. The datasets derived from the three cell lines are included in the appendix. These datasets provide new information about the oxysterol distribution in THP-1 monocytes and macrophages, SH-SY5Y cells and peripheral blood mononuclear cells. These datasets can be used as a guide for oxysterol distribution in the three cell lines for future studies, and can used for future method optimization, and for comparison of oxysterol recovery with other analytical techniques.

Original language	English
Article number	106382
Journal	Data in Brief
Volume	33
Early online date	16 Oct 2020
DOIs	https://doi.org/10.1016/j.dib.2020.106382
Publication status	Published - 1 Dec 2020

Bibliographical note

© 2020 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )

Funding: K Borah and HR Griffiths acknowledge INClusilver funded by the European Union, grant number H2020‐INNOSUP‐2017‐2017 731349; NeutroCure funded by the European Union, grant number H2020-FETOPEN-01-2018-2019-2020 861878 and Faculty Research Support Fund (FRSF) fund from the University of Surrey 2019–2020. IHKD acknowledges funding from Alzheimer's research UK midlands network grant 2019. AH Crosby and EL Baple acknowledge support from the Hereditary Spastic Paraplegia Support Group and The Diamond Jubilee Doctoral Scholarship Fund.

Keywords

Cholesterol
Liquid chromatography-mass spectrometry
Metabolism
Mitochondria
Oxysterol
Peripheral blood mononuclear cell

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10.1016/j.dib.2020.106382Licence: CC BY-NC-ND 3.0

Datasets of whole cell and mitochondrial oxysterol
© 2020 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
Final published version, 416 KBLicence: CC BY-NC-ND 3.0

Cite this

@article{c475da8853c44091bff5c824f87c7465,

title = "Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics",

abstract = "The raw datasets of oxysterol quantifications from whole cell and mitochondrial fractions of THP-1 monocytes and macrophages, neuronal-like SH-SH5Y cells and human peripheral blood mononuclear cells are presented. Oxysterols were quantified using a new liquid chromatography-mass spectrometry (LC-MS) and multiple reaction monitoring analysis published in the article “A quantitative LC-MS/MS method for analysis of mitochondrial-specific oxysterol metabolism” in Redox Biology [1]. This method showed improved extraction efficiency and recovery of mono and dihydroxycholesterols from cellular matrix. The datasets derived from the three cell lines are included in the appendix. These datasets provide new information about the oxysterol distribution in THP-1 monocytes and macrophages, SH-SY5Y cells and peripheral blood mononuclear cells. These datasets can be used as a guide for oxysterol distribution in the three cell lines for future studies, and can used for future method optimization, and for comparison of oxysterol recovery with other analytical techniques.",

keywords = "Cholesterol, Liquid chromatography-mass spectrometry, Metabolism, Mitochondria, Oxysterol, Peripheral blood mononuclear cell",

author = "Khushboo Borah and Rickman, {Olivia J.} and Nikol Voutsina and Baple, {Emma L.} and Dias, {Irundika HK} and Crosby, {Andrew H.} and Griffiths, {Helen R.}",

note = "{\textcopyright} 2020 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) Funding: K Borah and HR Griffiths acknowledge INClusilver funded by the European Union, grant number H2020‐INNOSUP‐2017‐2017 731349; NeutroCure funded by the European Union, grant number H2020-FETOPEN-01-2018-2019-2020 861878 and Faculty Research Support Fund (FRSF) fund from the University of Surrey 2019–2020. IHKD acknowledges funding from Alzheimer's research UK midlands network grant 2019. AH Crosby and EL Baple acknowledge support from the Hereditary Spastic Paraplegia Support Group and The Diamond Jubilee Doctoral Scholarship Fund.",

year = "2020",

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doi = "10.1016/j.dib.2020.106382",

language = "English",

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T1 - Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics

AU - Borah, Khushboo

AU - Rickman, Olivia J.

AU - Voutsina, Nikol

AU - Baple, Emma L.

AU - Dias, Irundika HK

AU - Crosby, Andrew H.

AU - Griffiths, Helen R.

N1 - © 2020 Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) Funding: K Borah and HR Griffiths acknowledge INClusilver funded by the European Union, grant number H2020‐INNOSUP‐2017‐2017 731349; NeutroCure funded by the European Union, grant number H2020-FETOPEN-01-2018-2019-2020 861878 and Faculty Research Support Fund (FRSF) fund from the University of Surrey 2019–2020. IHKD acknowledges funding from Alzheimer's research UK midlands network grant 2019. AH Crosby and EL Baple acknowledge support from the Hereditary Spastic Paraplegia Support Group and The Diamond Jubilee Doctoral Scholarship Fund.

PY - 2020/12/1

Y1 - 2020/12/1

N2 - The raw datasets of oxysterol quantifications from whole cell and mitochondrial fractions of THP-1 monocytes and macrophages, neuronal-like SH-SH5Y cells and human peripheral blood mononuclear cells are presented. Oxysterols were quantified using a new liquid chromatography-mass spectrometry (LC-MS) and multiple reaction monitoring analysis published in the article “A quantitative LC-MS/MS method for analysis of mitochondrial-specific oxysterol metabolism” in Redox Biology [1]. This method showed improved extraction efficiency and recovery of mono and dihydroxycholesterols from cellular matrix. The datasets derived from the three cell lines are included in the appendix. These datasets provide new information about the oxysterol distribution in THP-1 monocytes and macrophages, SH-SY5Y cells and peripheral blood mononuclear cells. These datasets can be used as a guide for oxysterol distribution in the three cell lines for future studies, and can used for future method optimization, and for comparison of oxysterol recovery with other analytical techniques.

AB - The raw datasets of oxysterol quantifications from whole cell and mitochondrial fractions of THP-1 monocytes and macrophages, neuronal-like SH-SH5Y cells and human peripheral blood mononuclear cells are presented. Oxysterols were quantified using a new liquid chromatography-mass spectrometry (LC-MS) and multiple reaction monitoring analysis published in the article “A quantitative LC-MS/MS method for analysis of mitochondrial-specific oxysterol metabolism” in Redox Biology [1]. This method showed improved extraction efficiency and recovery of mono and dihydroxycholesterols from cellular matrix. The datasets derived from the three cell lines are included in the appendix. These datasets provide new information about the oxysterol distribution in THP-1 monocytes and macrophages, SH-SY5Y cells and peripheral blood mononuclear cells. These datasets can be used as a guide for oxysterol distribution in the three cell lines for future studies, and can used for future method optimization, and for comparison of oxysterol recovery with other analytical techniques.

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KW - Liquid chromatography-mass spectrometry

KW - Metabolism

KW - Mitochondria

KW - Oxysterol

KW - Peripheral blood mononuclear cell

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DO - 10.1016/j.dib.2020.106382

M3 - Article

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JO - Data in Brief

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M1 - 106382

ER -

Datasets of whole cell and mitochondrial oxysterols derived from THP-1, SH-SY5Y and human peripheral blood mononuclear cells using targeted metabolomics

Abstract

Bibliographical note

Keywords

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