Decreased efficiency of trypsinization of cells following photodynamic therapy: evaluation of a role for tissue transglutaminase

Denise J. Ball, Stephen Mayhew, David I. Vernon, Martin Griffin, Stanley B. Brown

Research output: Contribution to journalArticle

Abstract

Identifying the cellular responses to photodynamic therapy (PDT) is important if the mechanisms of cellular damage are to be fully understood. The relationship between sensitizer, fluence rate and the removal of cells by trypsinization was studied using the RIF-1 cell line. Following treatment of RIF-1 cells with pyridinium zinc (II) phthalocyanine (PPC), or polyhaematoporphyrin at 10 mW cm−2 (3 J cm−2), there was a significant number of cells that were not removed by trypsin incubation compared to controls. Decreasing the fluence rate from 10 to 2.5 mW cm−2 resulted in a two-fold increase in the number of cells attached to the substratum when PPC used as sensitizer; however, with 5,10,15,20 meso-tetra(hydroxyphenyl) chlorin (m-THPC) there was no resistance to trypsinization following treatment at either fluence rate. The results indicate that resistance of cells to trypsinization following PDT is likely to be both sensitizer and fluence rate dependent. Increased activity of the enzyme tissue-transglutaminase (tTGase) was observed following PPC-PDT, but not following m-THPC-PDT. Similar results were obtained using HT29 human colonic carcinoma and ECV304 human umbilical vein endothelial cell lines. Hamster fibrosarcoma cell (Met B) clones transfected with human tTGase also exhibited resistance to trypsinization following PPC-mediated photosensitization; however, a similar degree of resistance was observed in PDT-treated control Met B cells suggesting that tTGase activity alone was not involved in this process.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalPhotochemistry and Photobiology
Volume73
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Photodynamic therapy
Photochemotherapy
therapy
evaluation
fluence
cells
cultured cells
Cell Count
Cells
Photosensitivity Disorders
Cell Line
Fibrosarcoma
Endothelial cells
Human Umbilical Vein Endothelial Cells
trypsin
hamsters
Cricetinae
Trypsin
veins
B-Lymphocytes

Cite this

@article{4c40d5a683784e339d07085a76646b2d,
title = "Decreased efficiency of trypsinization of cells following photodynamic therapy: evaluation of a role for tissue transglutaminase",
abstract = "Identifying the cellular responses to photodynamic therapy (PDT) is important if the mechanisms of cellular damage are to be fully understood. The relationship between sensitizer, fluence rate and the removal of cells by trypsinization was studied using the RIF-1 cell line. Following treatment of RIF-1 cells with pyridinium zinc (II) phthalocyanine (PPC), or polyhaematoporphyrin at 10 mW cm−2 (3 J cm−2), there was a significant number of cells that were not removed by trypsin incubation compared to controls. Decreasing the fluence rate from 10 to 2.5 mW cm−2 resulted in a two-fold increase in the number of cells attached to the substratum when PPC used as sensitizer; however, with 5,10,15,20 meso-tetra(hydroxyphenyl) chlorin (m-THPC) there was no resistance to trypsinization following treatment at either fluence rate. The results indicate that resistance of cells to trypsinization following PDT is likely to be both sensitizer and fluence rate dependent. Increased activity of the enzyme tissue-transglutaminase (tTGase) was observed following PPC-PDT, but not following m-THPC-PDT. Similar results were obtained using HT29 human colonic carcinoma and ECV304 human umbilical vein endothelial cell lines. Hamster fibrosarcoma cell (Met B) clones transfected with human tTGase also exhibited resistance to trypsinization following PPC-mediated photosensitization; however, a similar degree of resistance was observed in PDT-treated control Met B cells suggesting that tTGase activity alone was not involved in this process.",
author = "Ball, {Denise J.} and Stephen Mayhew and Vernon, {David I.} and Martin Griffin and Brown, {Stanley B.}",
note = "MEDLINE{\circledR} is the source for the MeSH terms of this document.",
year = "2001",
doi = "10.1562/0031-8655(2001)073<0047:DEOTOC>2.0.CO;2",
language = "English",
volume = "73",
pages = "47--53",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "1",

}

Decreased efficiency of trypsinization of cells following photodynamic therapy : evaluation of a role for tissue transglutaminase. / Ball, Denise J.; Mayhew, Stephen; Vernon, David I.; Griffin, Martin; Brown, Stanley B.

In: Photochemistry and Photobiology, Vol. 73, No. 1, 2001, p. 47-53.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Decreased efficiency of trypsinization of cells following photodynamic therapy

T2 - evaluation of a role for tissue transglutaminase

AU - Ball, Denise J.

AU - Mayhew, Stephen

AU - Vernon, David I.

AU - Griffin, Martin

AU - Brown, Stanley B.

N1 - MEDLINE® is the source for the MeSH terms of this document.

PY - 2001

Y1 - 2001

N2 - Identifying the cellular responses to photodynamic therapy (PDT) is important if the mechanisms of cellular damage are to be fully understood. The relationship between sensitizer, fluence rate and the removal of cells by trypsinization was studied using the RIF-1 cell line. Following treatment of RIF-1 cells with pyridinium zinc (II) phthalocyanine (PPC), or polyhaematoporphyrin at 10 mW cm−2 (3 J cm−2), there was a significant number of cells that were not removed by trypsin incubation compared to controls. Decreasing the fluence rate from 10 to 2.5 mW cm−2 resulted in a two-fold increase in the number of cells attached to the substratum when PPC used as sensitizer; however, with 5,10,15,20 meso-tetra(hydroxyphenyl) chlorin (m-THPC) there was no resistance to trypsinization following treatment at either fluence rate. The results indicate that resistance of cells to trypsinization following PDT is likely to be both sensitizer and fluence rate dependent. Increased activity of the enzyme tissue-transglutaminase (tTGase) was observed following PPC-PDT, but not following m-THPC-PDT. Similar results were obtained using HT29 human colonic carcinoma and ECV304 human umbilical vein endothelial cell lines. Hamster fibrosarcoma cell (Met B) clones transfected with human tTGase also exhibited resistance to trypsinization following PPC-mediated photosensitization; however, a similar degree of resistance was observed in PDT-treated control Met B cells suggesting that tTGase activity alone was not involved in this process.

AB - Identifying the cellular responses to photodynamic therapy (PDT) is important if the mechanisms of cellular damage are to be fully understood. The relationship between sensitizer, fluence rate and the removal of cells by trypsinization was studied using the RIF-1 cell line. Following treatment of RIF-1 cells with pyridinium zinc (II) phthalocyanine (PPC), or polyhaematoporphyrin at 10 mW cm−2 (3 J cm−2), there was a significant number of cells that were not removed by trypsin incubation compared to controls. Decreasing the fluence rate from 10 to 2.5 mW cm−2 resulted in a two-fold increase in the number of cells attached to the substratum when PPC used as sensitizer; however, with 5,10,15,20 meso-tetra(hydroxyphenyl) chlorin (m-THPC) there was no resistance to trypsinization following treatment at either fluence rate. The results indicate that resistance of cells to trypsinization following PDT is likely to be both sensitizer and fluence rate dependent. Increased activity of the enzyme tissue-transglutaminase (tTGase) was observed following PPC-PDT, but not following m-THPC-PDT. Similar results were obtained using HT29 human colonic carcinoma and ECV304 human umbilical vein endothelial cell lines. Hamster fibrosarcoma cell (Met B) clones transfected with human tTGase also exhibited resistance to trypsinization following PPC-mediated photosensitization; however, a similar degree of resistance was observed in PDT-treated control Met B cells suggesting that tTGase activity alone was not involved in this process.

UR - http://www.scopus.com/inward/record.url?scp=0035241557&partnerID=8YFLogxK

UR - http://www.bioone.org/doi/abs/10.1562/0031-8655%282001%29073%3C0047%3ADEOTOC%3E2.0.CO%3B2

U2 - 10.1562/0031-8655(2001)073<0047:DEOTOC>2.0.CO;2

DO - 10.1562/0031-8655(2001)073<0047:DEOTOC>2.0.CO;2

M3 - Article

AN - SCOPUS:0035241557

VL - 73

SP - 47

EP - 53

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 1

ER -