Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime

Keyphrases

• Culture Conditions 100%
• Bioreactor 100%
• Culture Influence 100%
• Pichia Pastoris 100%
• Recombinant Protein Production 100%
• Scalable Model 100%
• Design of Experiments Approach 66%
• Predictive Models 66%
• Culture Media 33%
• Protein Production 33%
• Green Fluorescent Protein 33%
• Postgenomics 33%
• Host Metabolism 33%
• Protein Yield 33%
• Trial-and-error 33%
• Methanol 33%
• High Yielding 33%
• Shake Flask 33%
• Development Time 33%
• Recombinant Protein 33%
• Error Optimization 33%
• Genomic Sciences 33%
• Culture Volume 33%
• Fed-batch 33%
• Mini-bioreactors 33%
• Working Volume 33%
• Accumulation Model 33%
• Biomass Accumulation 33%
• Dissolved Oxygen 33%
• Microwell Plate 33%
• Induction Period 33%
• Predicted Yield 33%
• Pre-induction 33%
• Process Conditions 33%
• Process Development 33%
• Medium Composition 33%
• Multi-well 33%
• Oxygen Transfer Rate 33%
• Yield Improvement 33%
• Feed Rate 33%
• Production Stage 33%
• Stepwise Approach 33%
• Culture Density 33%

Immunology and Microbiology

• Bioreactor 100%
• Komagataella pastoris 100%
• Scale up 25%
• Process Development 25%

Chemical Engineering

• Biochemical Reactor 100%
• Methanol 25%
• Scalability 25%
• Dissolved Oxygen 25%