Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Daniel J. Kirby; Randip Kaur; Else M. Agger; Peter Andersen; Vincent W. Bramwell; Yvonne Perrie

doi:10.2174/1567201811310030003

Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Daniel J. Kirby, Randip Kaur, Else M. Agger, Peter Andersen, Vincent W. Bramwell, Yvonne Perrie

Research output: Contribution to journal › Article › peer-review

Abstract

This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

Original language	English
Pages (from-to)	268-278
Number of pages	11
Journal	Current Drug Delivery
Volume	11
Issue number	6
Early online date	4 Feb 2013
DOIs	https://doi.org/10.2174/1567201811310030003
Publication status	Published - 2013

Bibliographical note

This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no.
460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.

Keywords

adjuvant
DDA
ESEM
microspheres
PLGA
subunit vaccine

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Developing solid particulate vaccine adjuvantsAccepted author manuscript, 427 KB

http://benthamscience.com/journal/abstracts.php?journalID=cdd&articleID=110594

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@article{39036dc1f6ed49aca855b1a1dc0af9be,

title = "Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity",

abstract = "This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.",

keywords = "adjuvant, DDA, ESEM, microspheres, PLGA, subunit vaccine",

author = "Kirby, {Daniel J.} and Randip Kaur and Agger, {Else M.} and Peter Andersen and Bramwell, {Vincent W.} and Yvonne Perrie",

note = "This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no. 460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.",

year = "2013",

doi = "10.2174/1567201811310030003",

language = "English",

volume = "11",

pages = "268--278",

journal = "Current Drug Delivery",

issn = "1567-2018",

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AU - Kaur, Randip

AU - Agger, Else M.

AU - Andersen, Peter

AU - Bramwell, Vincent W.

AU - Perrie, Yvonne

N1 - This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no. 460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.

PY - 2013

Y1 - 2013

N2 - This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

AB - This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

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KW - ESEM

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SN - 1567-2018

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JO - Current Drug Delivery

JF - Current Drug Delivery

IS - 6

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Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Abstract

Bibliographical note

Keywords

Access to Document

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Fingerprint

Formulation and characterisation of an effective particulate delivery vehicle for the novel sub-unit vaccine antigen, Ag85B-ESAT-6

Cite this

Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Student theses

Formulation and characterisation of an effective particulate delivery vehicle for the novel sub-unit vaccine antigen, Ag85B-ESAT-6

Cite this