Developing solid particulate vaccine adjuvants: surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity

Daniel J. Kirby, Randip Kaur, Else M. Agger, Peter Andersen, Vincent W. Bramwell, Yvonne Perrie

Research output: Contribution to journalArticle

Abstract

This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.
LanguageEnglish
Pages268-278
Number of pages11
JournalCurrent Drug Delivery
Volume11
Issue number6
Early online date4 Feb 2013
DOIs
Publication statusPublished - 2013

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Surface Antigens
Cellular Immunity
Vaccines
Antigens
Microspheres
Emulsions
Polyglactin 910
Antigen Presentation
Chitosan

Bibliographical note

This study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no.
460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.

Keywords

  • adjuvant
  • DDA
  • ESEM
  • microspheres
  • PLGA
  • subunit vaccine

Cite this

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abstract = "This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.",
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Developing solid particulate vaccine adjuvants : surface bound antigen favouring a humoural response, whereas entrapped antigen shows a tendency for cell mediated immunity. / Kirby, Daniel J.; Kaur, Randip; Agger, Else M.; Andersen, Peter; Bramwell, Vincent W.; Perrie, Yvonne.

In: Current Drug Delivery, Vol. 11, No. 6, 2013, p. 268-278.

Research output: Contribution to journalArticle

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