This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-co-glycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.
|Number of pages||11|
|Journal||Current Drug Delivery|
|Early online date||4 Feb 2013|
|Publication status||Published - 2013|
Bibliographical noteThis study was funded by the European Commission 459 (contract no. LSHP-CT-2003-503367). This work was also part funded by NewTBVAC (contract no.
460 HEALTH-F3-2009-241745). NewTBVAC has been made possible by contributions from the European 461 Commission.
- subunit vaccine
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Formulation and characterisation of an effective particulate delivery vehicle for the novel sub-unit vaccine antigen, Ag85B-ESAT-6Author: Kirby, D. J., Jul 2007
Supervisor: Perrie, Y. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of PhilosophyFile