Development and Characterization of Gallium-Doped Bioactive Glasses for Potential Bone Cancer Applications

Karan S. Rana; Lucas Pereira De Souza; Mark A. Isaacs; Farah N. S. Raja; Alexander P. Morrell; Richard A Martin

doi:10.1021/acsbiomaterials.7b00283

Development and Characterization of Gallium-Doped Bioactive Glasses for Potential Bone Cancer Applications

Karan S. Rana, Lucas Pereira De Souza, Mark A. Isaacs, Farah N. S. Raja, Alexander P. Morrell, Richard A Martin

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, we have developed a series of novel gallium oxide doped bioactive glasses to specifically target osteosarcoma cells while aiding new bone formation. The results show that osteosarcoma (Saos-2) cell death is induced through the addition of gallium oxide. Relative to the gallium-free control glass (0% Ga) glasses containing 1, 2, and 3% Ga decreased Saos-2 cell viability in a dose dependent manner. After 72 h in media preconditioned with 3% Ga Saos-2 cell viability was reduced by over 50%. Corresponding studies undertaken on primary normal human osteoblast cells (NHOst) demonstrated no adverse effects to the gallium containing glasses. Hydroxyapatite formation was observed for all glasses when exposed to simulated body fluid.

Original language	English
Pages (from-to)	3425-3432
Journal	ACS Biomaterials Science and Engineering
Volume	3
Issue number	12
Early online date	22 Sept 2017
DOIs	https://doi.org/10.1021/acsbiomaterials.7b00283
Publication status	Published - 11 Dec 2017

Bibliographical note

Copyright © 2017 American Chemical Society. This is the accepted author manuscript.

Funding: Osteosarcoma UK.

Keywords

apatite; bioactive glass; bone; cancer; gallium; sarcoma

Access to Document

10.1021/acsbiomaterials.7b00283

The development and characterisation of gallium doped
Copyright © 2017 American Chemical Society. This is the accepted author manuscript.
Accepted author manuscript, 2.72 MBLicence: CC BY-NC 3.0

Cite this

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title = "Development and Characterization of Gallium-Doped Bioactive Glasses for Potential Bone Cancer Applications",

abstract = "In this study, we have developed a series of novel gallium oxide doped bioactive glasses to specifically target osteosarcoma cells while aiding new bone formation. The results show that osteosarcoma (Saos-2) cell death is induced through the addition of gallium oxide. Relative to the gallium-free control glass (0% Ga) glasses containing 1, 2, and 3% Ga decreased Saos-2 cell viability in a dose dependent manner. After 72 h in media preconditioned with 3% Ga Saos-2 cell viability was reduced by over 50%. Corresponding studies undertaken on primary normal human osteoblast cells (NHOst) demonstrated no adverse effects to the gallium containing glasses. Hydroxyapatite formation was observed for all glasses when exposed to simulated body fluid.",

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note = "Copyright {\textcopyright} 2017 American Chemical Society. This is the accepted author manuscript. Funding: Osteosarcoma UK.",

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AU - Rana, Karan S.

AU - Souza, Lucas Pereira De

AU - Isaacs, Mark A.

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AU - Morrell, Alexander P.

AU - Martin, Richard A

PY - 2017/12/11

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N2 - In this study, we have developed a series of novel gallium oxide doped bioactive glasses to specifically target osteosarcoma cells while aiding new bone formation. The results show that osteosarcoma (Saos-2) cell death is induced through the addition of gallium oxide. Relative to the gallium-free control glass (0% Ga) glasses containing 1, 2, and 3% Ga decreased Saos-2 cell viability in a dose dependent manner. After 72 h in media preconditioned with 3% Ga Saos-2 cell viability was reduced by over 50%. Corresponding studies undertaken on primary normal human osteoblast cells (NHOst) demonstrated no adverse effects to the gallium containing glasses. Hydroxyapatite formation was observed for all glasses when exposed to simulated body fluid.

AB - In this study, we have developed a series of novel gallium oxide doped bioactive glasses to specifically target osteosarcoma cells while aiding new bone formation. The results show that osteosarcoma (Saos-2) cell death is induced through the addition of gallium oxide. Relative to the gallium-free control glass (0% Ga) glasses containing 1, 2, and 3% Ga decreased Saos-2 cell viability in a dose dependent manner. After 72 h in media preconditioned with 3% Ga Saos-2 cell viability was reduced by over 50%. Corresponding studies undertaken on primary normal human osteoblast cells (NHOst) demonstrated no adverse effects to the gallium containing glasses. Hydroxyapatite formation was observed for all glasses when exposed to simulated body fluid.

KW - apatite; bioactive glass; bone; cancer; gallium; sarcoma

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Development and Characterization of Gallium-Doped Bioactive Glasses for Potential Bone Cancer Applications

Abstract

Bibliographical note

Keywords

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