Development, characterisation and biocompatibility testing of a cobalt-containing titanium phosphate-based glass for engineering of vascularized hard tissues

In-Ho Lee, Hye-sun Yu, Nilay J Lakhkar, Hae-Won Kim, Myoung-Seon Gong, Jonathan C Knowles, Ivan B Wall

Research output: Contribution to journalArticle

Abstract

There is a continuing need to develop scaffold materials that can promote vascularisation throughout the tissue engineered construct. This study investigated the effect of cobalt oxide (CoO) doped into titanium phosphate glasses on material properties, biocompatibility and vascular endothelial growth factor (VEGF) secretion by osteoblastic MG63 cells. Glasses composed of (P2O5)45(Na2O)20(TiO2)05(CaO)30-x(CoO)x(x=0, 5, 10, and 15 mol%) were fabricated and the effect of Co on physicochemical properties including density, glass transition temperature (Tg), degradation rate, ion release, and pH changes was assessed. The results showed that incorporation of CoO into the glass system produced an increase in density with little change in Tg. It was then confirmed that the pH did not change significantly when CoO was incorporated in the glass, and stayed constant at around 6.5-7.0 throughout the dissolution study period of 336 h. Ion release results followed a specific pattern with increasing amounts of CoO. In general, although incorporation of CoO into a titanium phosphate glass increased its density, other bulk and surface properties of the glass did not show any significant changes. Cell culture studies performed using MG63 cells over a 7-day period indicated that the glasses provide a stable surface for cell attachment and are biocompatible. Furthermore, VEGF secretion was significantly enhanced on all glasses compared with standard tissue culture plastic and Co doping enhanced this effect further. In conclusion, the developed Co-doped glasses are stable and biocompatible and thus offer enhanced potential for engineering vascularized tissue.

Original languageEnglish
Pages (from-to)2104-2112
Number of pages9
JournalMaterials Science and Engineering C
Volume33
Issue number4
DOIs
Publication statusPublished - 1 May 2013

Fingerprint

biocompatibility
Cobalt
Biocompatibility
phosphates
Phosphates
cobalt oxides
cobalt
titanium
Titanium
engineering
Tissue
Glass
glass
Testing
Oxides
secretions
phosphorus pentoxide
Vascular Endothelial Growth Factor A
cells
Ions

Keywords

  • Animals
  • Cell Line, Tumor
  • Cell Proliferation/drug effects
  • Cobalt/pharmacology
  • Glass/chemistry
  • Humans
  • Hydrogen-Ion Concentration/drug effects
  • Ions
  • Materials Testing/methods
  • Neovascularization, Physiologic/drug effects
  • Osteoblasts/cytology
  • Rats
  • Spectroscopy, Fourier Transform Infrared
  • Tissue Engineering/methods
  • Titanium/pharmacology
  • Transition Temperature/drug effects
  • Vascular Endothelial Growth Factor A/metabolism
  • X-Ray Diffraction

Cite this

Lee, In-Ho ; Yu, Hye-sun ; Lakhkar, Nilay J ; Kim, Hae-Won ; Gong, Myoung-Seon ; Knowles, Jonathan C ; Wall, Ivan B. / Development, characterisation and biocompatibility testing of a cobalt-containing titanium phosphate-based glass for engineering of vascularized hard tissues. In: Materials Science and Engineering C. 2013 ; Vol. 33, No. 4. pp. 2104-2112.
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abstract = "There is a continuing need to develop scaffold materials that can promote vascularisation throughout the tissue engineered construct. This study investigated the effect of cobalt oxide (CoO) doped into titanium phosphate glasses on material properties, biocompatibility and vascular endothelial growth factor (VEGF) secretion by osteoblastic MG63 cells. Glasses composed of (P2O5)45(Na2O)20(TiO2)05(CaO)30-x(CoO)x(x=0, 5, 10, and 15 mol{\%}) were fabricated and the effect of Co on physicochemical properties including density, glass transition temperature (Tg), degradation rate, ion release, and pH changes was assessed. The results showed that incorporation of CoO into the glass system produced an increase in density with little change in Tg. It was then confirmed that the pH did not change significantly when CoO was incorporated in the glass, and stayed constant at around 6.5-7.0 throughout the dissolution study period of 336 h. Ion release results followed a specific pattern with increasing amounts of CoO. In general, although incorporation of CoO into a titanium phosphate glass increased its density, other bulk and surface properties of the glass did not show any significant changes. Cell culture studies performed using MG63 cells over a 7-day period indicated that the glasses provide a stable surface for cell attachment and are biocompatible. Furthermore, VEGF secretion was significantly enhanced on all glasses compared with standard tissue culture plastic and Co doping enhanced this effect further. In conclusion, the developed Co-doped glasses are stable and biocompatible and thus offer enhanced potential for engineering vascularized tissue.",
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Development, characterisation and biocompatibility testing of a cobalt-containing titanium phosphate-based glass for engineering of vascularized hard tissues. / Lee, In-Ho; Yu, Hye-sun; Lakhkar, Nilay J; Kim, Hae-Won; Gong, Myoung-Seon; Knowles, Jonathan C; Wall, Ivan B.

In: Materials Science and Engineering C, Vol. 33, No. 4, 01.05.2013, p. 2104-2112.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development, characterisation and biocompatibility testing of a cobalt-containing titanium phosphate-based glass for engineering of vascularized hard tissues

AU - Lee, In-Ho

AU - Yu, Hye-sun

AU - Lakhkar, Nilay J

AU - Kim, Hae-Won

AU - Gong, Myoung-Seon

AU - Knowles, Jonathan C

AU - Wall, Ivan B

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AB - There is a continuing need to develop scaffold materials that can promote vascularisation throughout the tissue engineered construct. This study investigated the effect of cobalt oxide (CoO) doped into titanium phosphate glasses on material properties, biocompatibility and vascular endothelial growth factor (VEGF) secretion by osteoblastic MG63 cells. Glasses composed of (P2O5)45(Na2O)20(TiO2)05(CaO)30-x(CoO)x(x=0, 5, 10, and 15 mol%) were fabricated and the effect of Co on physicochemical properties including density, glass transition temperature (Tg), degradation rate, ion release, and pH changes was assessed. The results showed that incorporation of CoO into the glass system produced an increase in density with little change in Tg. It was then confirmed that the pH did not change significantly when CoO was incorporated in the glass, and stayed constant at around 6.5-7.0 throughout the dissolution study period of 336 h. Ion release results followed a specific pattern with increasing amounts of CoO. In general, although incorporation of CoO into a titanium phosphate glass increased its density, other bulk and surface properties of the glass did not show any significant changes. Cell culture studies performed using MG63 cells over a 7-day period indicated that the glasses provide a stable surface for cell attachment and are biocompatible. Furthermore, VEGF secretion was significantly enhanced on all glasses compared with standard tissue culture plastic and Co doping enhanced this effect further. In conclusion, the developed Co-doped glasses are stable and biocompatible and thus offer enhanced potential for engineering vascularized tissue.

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KW - Hydrogen-Ion Concentration/drug effects

KW - Ions

KW - Materials Testing/methods

KW - Neovascularization, Physiologic/drug effects

KW - Osteoblasts/cytology

KW - Rats

KW - Spectroscopy, Fourier Transform Infrared

KW - Tissue Engineering/methods

KW - Titanium/pharmacology

KW - Transition Temperature/drug effects

KW - Vascular Endothelial Growth Factor A/metabolism

KW - X-Ray Diffraction

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U2 - 10.1016/j.msec.2013.01.024

DO - 10.1016/j.msec.2013.01.024

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