Development of a co-cultured skin model using biocomposite cell support membranes

Niann-Tzyy Dai; Chiao-Hsi Chiang; Ming-Kung Yeh; David Demeral Liu; Eric F. Adams; Allan G.A. Coombes

Development of a co-cultured skin model using biocomposite cell support membranes

Niann-Tzyy Dai, Chiao-Hsi Chiang, Ming-Kung Yeh, David Demeral Liu, Eric F. Adams, Allan G.A. Coombes

School of Biosciences

Research output: Chapter in Book/Published conference output › Conference publication

Abstract

The development of a co-cultured skin model was analyzed using biocomposite cell support membranes. 1:20 w/w collagen:poly(ε-caprolactone) (PCL) composite membranes were prepared by impregnation of lyophilised collagen mats with a solution of PCL (MW 115,000) in DCM followed by solvent evaporation (3). A sterilizing filter holder with a diameter of 13 mm was modified to hold the biocomposite film between open chambers, which allowed the cell culture medium to contact both sides of the membrane. The results show attachment and spreading of both cell types indicating the establishment of focal contacts with the collagen: PCL substrate.

Original language	English
Title of host publication	Transactions - 7th World Biomaterials Congress
Publication status	Published - 1 Dec 2004
Event	Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: 17 May 2004 → 21 May 2004

Conference

Conference	Transactions - 7th World Biomaterials Congress
Country/Territory	Australia
City	Sydney
Period	17/05/04 → 21/05/04

Cite this

@inproceedings{ad41bf9602264dd28896c215e0de271c,

title = "Development of a co-cultured skin model using biocomposite cell support membranes",

abstract = "The development of a co-cultured skin model was analyzed using biocomposite cell support membranes. 1:20 w/w collagen:poly(ε-caprolactone) (PCL) composite membranes were prepared by impregnation of lyophilised collagen mats with a solution of PCL (MW 115,000) in DCM followed by solvent evaporation (3). A sterilizing filter holder with a diameter of 13 mm was modified to hold the biocomposite film between open chambers, which allowed the cell culture medium to contact both sides of the membrane. The results show attachment and spreading of both cell types indicating the establishment of focal contacts with the collagen: PCL substrate.",

author = "Niann-Tzyy Dai and Chiao-Hsi Chiang and Ming-Kung Yeh and Liu, {David Demeral} and Adams, {Eric F.} and Coombes, {Allan G.A.}",

year = "2004",

month = dec,

day = "1",

language = "English",

isbn = "1877040193",

booktitle = "Transactions - 7th World Biomaterials Congress",

note = "Transactions - 7th World Biomaterials Congress ; Conference date: 17-05-2004 Through 21-05-2004",

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TY - GEN

T1 - Development of a co-cultured skin model using biocomposite cell support membranes

AU - Dai, Niann-Tzyy

AU - Chiang, Chiao-Hsi

AU - Yeh, Ming-Kung

AU - Liu, David Demeral

AU - Adams, Eric F.

AU - Coombes, Allan G.A.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - The development of a co-cultured skin model was analyzed using biocomposite cell support membranes. 1:20 w/w collagen:poly(ε-caprolactone) (PCL) composite membranes were prepared by impregnation of lyophilised collagen mats with a solution of PCL (MW 115,000) in DCM followed by solvent evaporation (3). A sterilizing filter holder with a diameter of 13 mm was modified to hold the biocomposite film between open chambers, which allowed the cell culture medium to contact both sides of the membrane. The results show attachment and spreading of both cell types indicating the establishment of focal contacts with the collagen: PCL substrate.

AB - The development of a co-cultured skin model was analyzed using biocomposite cell support membranes. 1:20 w/w collagen:poly(ε-caprolactone) (PCL) composite membranes were prepared by impregnation of lyophilised collagen mats with a solution of PCL (MW 115,000) in DCM followed by solvent evaporation (3). A sterilizing filter holder with a diameter of 13 mm was modified to hold the biocomposite film between open chambers, which allowed the cell culture medium to contact both sides of the membrane. The results show attachment and spreading of both cell types indicating the establishment of focal contacts with the collagen: PCL substrate.

UR - http://www.scopus.com/inward/record.url?scp=13844255651&partnerID=8YFLogxK

M3 - Conference publication

AN - SCOPUS:13844255651

SN - 1877040193

SN - 9781877040191

BT - Transactions - 7th World Biomaterials Congress

T2 - Transactions - 7th World Biomaterials Congress

Y2 - 17 May 2004 through 21 May 2004

ER -

Development of a co-cultured skin model using biocomposite cell support membranes

Abstract

Conference

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