The development of a co-cultured skin model was analyzed using biocomposite cell support membranes. 1:20 w/w collagen:poly(ε-caprolactone) (PCL) composite membranes were prepared by impregnation of lyophilised collagen mats with a solution of PCL (MW 115,000) in DCM followed by solvent evaporation (3). A sterilizing filter holder with a diameter of 13 mm was modified to hold the biocomposite film between open chambers, which allowed the cell culture medium to contact both sides of the membrane. The results show attachment and spreading of both cell types indicating the establishment of focal contacts with the collagen: PCL substrate.
|Title of host publication||Transactions - 7th World Biomaterials Congress|
|Publication status||Published - 1 Dec 2004|
|Event||Transactions - 7th World Biomaterials Congress - Sydney, Australia|
Duration: 17 May 2004 → 21 May 2004
|Conference||Transactions - 7th World Biomaterials Congress|
|Period||17/05/04 → 21/05/04|