Development of a co-cultured skin model using biocomposite cell support membranes

Niann-Tzyy Dai, Chiao-Hsi Chiang, Ming-Kung Yeh, David Demeral Liu, Eric F. Adams, Allan G.A. Coombes

Research output: Chapter in Book/Report/Conference proceedingConference publication

Abstract

The development of a co-cultured skin model was analyzed using biocomposite cell support membranes. 1:20 w/w collagen:poly(ε-caprolactone) (PCL) composite membranes were prepared by impregnation of lyophilised collagen mats with a solution of PCL (MW 115,000) in DCM followed by solvent evaporation (3). A sterilizing filter holder with a diameter of 13 mm was modified to hold the biocomposite film between open chambers, which allowed the cell culture medium to contact both sides of the membrane. The results show attachment and spreading of both cell types indicating the establishment of focal contacts with the collagen: PCL substrate.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Publication statusPublished - 1 Dec 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, United Kingdom
Duration: 17 May 200421 May 2004

Conference

ConferenceTransactions - 7th World Biomaterials Congress
CountryUnited Kingdom
CitySydney
Period17/05/0421/05/04

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