Within this work, we develop vesicles incorporating sub-unit antigens as solid dosage forms suitable for the oral delivery of vaccines. Using a combination of trehalose, dextran and mannitol, freeze-dried oral disintegrating tablets were formed which upon rehydration release bilayer vesicles incorporating antigen. Initial studies focused on the optimisation of the freeze-dry cycle and subsequently excipient content was optimised by testing tablet hardness, disintegration time and moisture content. The use of 10% mannitol and 10% dextran produced durable tablets which offered strong resistance to mechanical damage yet appropriate disintegration times and dispersed to release niosomes-entrapping antigen. From these studies, we have formulated a bilayer vesicle vaccine delivery system as rapid disintegrating tablets and capsules.
Bibliographical note© 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Funding: BBSRC Industrial Case studentship (BB/G017948/1).
- oral vaccines
- oral disintegrating tablets
Wilkhu, J. S., Mcneil, S. E., Anderson, D. E., Kirchmeier, M., & Perrie, Y. (2017). Development of a solid dosage platform for the oral delivery of bilayer vesicles. European Journal of Pharmaceutical Sciences, in press. https://doi.org/10.1016/j.ejps.2017.06.014