TY - JOUR
T1 - Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling
AU - Alea, Mileidys Perez
AU - Borroto-Escuela, Dasiel O.
AU - Romero-Fernandez, Wilber
AU - Fuxe, Kjell
AU - Garriga, Pere
PY - 2011/8/15
Y1 - 2011/8/15
N2 - Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M3, M4, and M5 subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M3 subtype in a Gq/11-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M3 and M5 both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.
AB - Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M3, M4, and M5 subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M3 subtype in a Gq/11-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M3 and M5 both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.
KW - G protein coupled receptors
KW - Immunopharmacology
KW - Lymphocytes
KW - Muscarinic acetylcholine receptors
KW - Signal transduction
UR - http://www.scopus.com/inward/record.url?scp=80051474913&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2011.05.010
DO - 10.1016/j.jneuroim.2011.05.010
M3 - Article
C2 - 21742386
AN - SCOPUS:80051474913
SN - 0165-5728
VL - 237
SP - 13
EP - 22
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -