Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling

Mileidys Perez Alea; Dasiel O. Borroto-Escuela; Wilber Romero-Fernandez; Kjell Fuxe; Pere Garriga

doi:10.1016/j.jneuroim.2011.05.010

Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling

Mileidys Perez Alea^*
, Dasiel O. Borroto-Escuela
, Wilber Romero-Fernandez
, Kjell Fuxe
, Pere Garriga

^*Corresponding author for this work

College of Health and Life Sciences

Universitat Politècnica de Catalunya
Aston University
Karolinska Institutet

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M₃, M₄, and M₅ subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M₃ subtype in a G_q/11-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M₃ and M₅ both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.

Original language	English
Pages (from-to)	13-22
Number of pages	10
Journal	Journal of Neuroimmunology
Volume	237
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2011.05.010
Publication status	Published - 15 Aug 2011

Keywords

G protein coupled receptors
Immunopharmacology
Lymphocytes
Muscarinic acetylcholine receptors
Signal transduction

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10.1016/j.jneuroim.2011.05.010

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title = "Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling",

abstract = "Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M3, M4, and M5 subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M3 subtype in a Gq/11-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M3 and M5 both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.",

keywords = "G protein coupled receptors, Immunopharmacology, Lymphocytes, Muscarinic acetylcholine receptors, Signal transduction",

author = "Alea, \{Mileidys Perez\} and Borroto-Escuela, \{Dasiel O.\} and Wilber Romero-Fernandez and Kjell Fuxe and Pere Garriga",

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AU - Alea, Mileidys Perez

AU - Borroto-Escuela, Dasiel O.

AU - Romero-Fernandez, Wilber

AU - Fuxe, Kjell

AU - Garriga, Pere

PY - 2011/8/15

Y1 - 2011/8/15

N2 - Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M3, M4, and M5 subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M3 subtype in a Gq/11-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M3 and M5 both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.

AB - Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M3, M4, and M5 subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M3 subtype in a Gq/11-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M3 and M5 both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.

KW - G protein coupled receptors

KW - Immunopharmacology

KW - Lymphocytes

KW - Muscarinic acetylcholine receptors

KW - Signal transduction

UR - https://www.scopus.com/pages/publications/80051474913

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IS - 1-2

ER -

Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling

Abstract

Keywords

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