Discrete store-operated calcium influx into an intracellular compartment in rabbit arteriolar smooth muscle

R. Flemming, A. Cheong, A. M. Dedman, D. J. Beech*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This study tested the hypothesis that store-operated channels (SOCs) exist as a discrete population of Ca2+ channels activated by depletion of intracellular Ca2+ stores in cerebral arteriolar smooth muscle cells and explored their direct contractile function. Using the Ca2+ indicator fura-PE3 it was observed that depletion of sarcoplasmic reticulum (SR) Ca2+ by inhibition of SR Ca2+-ATPase (SERCA) led to sustained elevation of [Ca2+]i that depended on extracellular Ca2+ and slightly enhanced Mn2+ entry. Enhanced background Ca2+ influx did not explain the raised [Ca2+]i in response to SERCA inhibitors because it had marked gadolinium (Gd3+) sensitivity, which background pathways did not. Effects were not secondary to changes in membrane potential. Thus SR Ca2+ depletion activated SOCs. Strikingly, SOC-mediated Ca2+ influx did not evoke constriction of the arterioles, which were in a resting state. This was despite the fura-PE3-indicated [Ca2+]i rise being greater than that evoked by 20 mM [K+]o (which did cause constriction). Release of endothelial vasodilators did not explain the absence of SOC-mediated constriction, nor did a change in Ca2+ sensitivity of the contractile proteins. We suggest SOCs are a discrete subset of Ca2+ channels allowing Ca2+ influx into a 'non-contractile' compartment in cerebral arteriolar smooth muscle cells.

Original languageEnglish
Pages (from-to)455-464
Number of pages10
JournalJournal of Physiology
Volume543
Issue number2
DOIs
Publication statusPublished - 1 Sep 2002

Fingerprint

Dive into the research topics of 'Discrete store-operated calcium influx into an intracellular compartment in rabbit arteriolar smooth muscle'. Together they form a unique fingerprint.

Cite this