DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre

Monika Rutowska*, Jiadi Lu, Fatima C. Garcia-Gunning, Andrew D. Ellis

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference publication

Abstract

In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.

Original languageEnglish
Title of host publication21st International Conference on Optical Fiber Sensors
PublisherSPIE
ISBN (Print)9780819482464
DOIs
Publication statusPublished - 9 Jun 2011
Event21st International Conference on Optical Fiber Sensors - Ottawa, ON, Canada
Duration: 15 May 201119 May 2011
http://www.ofs21.org/

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7753
ISSN (Print)0277-786X

Conference

Conference21st International Conference on Optical Fiber Sensors
Abbreviated titleOFS21
CountryCanada
CityOttawa, ON
Period15/05/1119/05/11
Internet address

Bibliographical note

Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

  • biosensing
  • DNA
  • fluorescence
  • hollow core
  • hydrogel
  • Photonic crystal fibres
  • silanisation

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