In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.
|Title of host publication||21st International Conference on Optical Fiber Sensors|
|Publication status||Published - 9 Jun 2011|
|Event||21st International Conference on Optical Fiber Sensors - Ottawa, ON, Canada|
Duration: 15 May 2011 → 19 May 2011
|Name||Proceedings of SPIE - The International Society for Optical Engineering|
|Conference||21st International Conference on Optical Fiber Sensors|
|Period||15/05/11 → 19/05/11|
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- hollow core
- Photonic crystal fibres