DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre

Monika Rutowska; Jiadi Lu; Fatima C. Garcia-Gunning; Andrew D. Ellis

doi:10.1117/12.886070

DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre

Monika Rutowska^*, Jiadi Lu, Fatima C. Garcia-Gunning, Andrew D. Ellis

^*Corresponding author for this work

Electrical and Electronic Engineering

Research output: Chapter in Book/Published conference output › Conference publication

Abstract

In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.

Original language	English
Title of host publication	21st International Conference on Optical Fiber Sensors
Publisher	SPIE
ISBN (Print)	9780819482464
DOIs	https://doi.org/10.1117/12.886070
Publication status	Published - 9 Jun 2011
Event	21st International Conference on Optical Fiber Sensors - Ottawa, ON, Canada Duration: 15 May 2011 → 19 May 2011 http://www.ofs21.org/

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7753
ISSN (Print)	0277-786X

Conference

Conference	21st International Conference on Optical Fiber Sensors
Abbreviated title	OFS21
Country/Territory	Canada
City	Ottawa, ON
Period	15/05/11 → 19/05/11
Internet address	http://www.ofs21.org/

Bibliographical note

Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

biosensing
DNA
fluorescence
hollow core
hydrogel
Photonic crystal fibres
silanisation

Access to Document

10.1117/12.886070

DNA probe detection
Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
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Cite this

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title = "DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre",

abstract = "In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.",

keywords = "biosensing, DNA, fluorescence, hollow core, hydrogel, Photonic crystal fibres, silanisation",

author = "Monika Rutowska and Jiadi Lu and Garcia-Gunning, {Fatima C.} and Ellis, {Andrew D.}",

note = "Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. ; 21st International Conference on Optical Fiber Sensors, OFS21 ; Conference date: 15-05-2011 Through 19-05-2011",

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doi = "10.1117/12.886070",

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series = "Proceedings of SPIE - The International Society for Optical Engineering",

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Rutowska, M, Lu, J, Garcia-Gunning, FC & Ellis, AD 2011, DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre. in 21st International Conference on Optical Fiber Sensors., 77536I, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7753, SPIE, 21st International Conference on Optical Fiber Sensors, Ottawa, ON, Canada, 15/05/11. https://doi.org/10.1117/12.886070

DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre. / Rutowska, Monika; Lu, Jiadi; Garcia-Gunning, Fatima C. et al.

21st International Conference on Optical Fiber Sensors. SPIE, 2011. 77536I (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7753).

Research output: Chapter in Book/Published conference output › Conference publication

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AU - Rutowska, Monika

AU - Lu, Jiadi

AU - Garcia-Gunning, Fatima C.

AU - Ellis, Andrew D.

N1 - Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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N2 - In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.

AB - In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.

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KW - hydrogel

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DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre

Abstract

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Bibliographical note

Keywords

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