Abstract
In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.
| Original language | English |
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| Title of host publication | 21st International Conference on Optical Fiber Sensors |
| Publisher | Society of Photo-Optical Instrumentation Engineers (SPIE) |
| ISBN (Print) | 9780819482464 |
| DOIs | |
| Publication status | Published - 9 Jun 2011 |
| Event | 21st International Conference on Optical Fiber Sensors - Ottawa, ON, Canada Duration: 15 May 2011 → 19 May 2011 http://www.ofs21.org/ |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
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| Volume | 7753 |
| ISSN (Print) | 0277-786X |
Conference
| Conference | 21st International Conference on Optical Fiber Sensors |
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| Abbreviated title | OFS21 |
| Country/Territory | Canada |
| City | Ottawa, ON |
| Period | 15/05/11 → 19/05/11 |
| Internet address |
Bibliographical note
Copyright 2011 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.Keywords
- biosensing
- DNA
- fluorescence
- hollow core
- hydrogel
- Photonic crystal fibres
- silanisation