DNA-templated protein arrays for single-molecule imaging

Daniele N. Selmi, Roslin J. Adamson, Helen Attrill, Alan D. Goddard, Robert J.C. Gilbert, Anthony Watts, Andrew J. Turberfield

Research output: Contribution to journalLetter

Abstract

Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

Original languageEnglish
Pages (from-to)657-660
Number of pages4
JournalNano Letters
Volume11
Issue number2
Early online date10 Jan 2011
DOIs
Publication statusPublished - 9 Feb 2011

Keywords

  • DNA templates
  • G-protein-coupled membrane receptor
  • nanoaffinity
  • nanostructure
  • single-particle electron cryomicrosopy

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    Selmi, D. N., Adamson, R. J., Attrill, H., Goddard, A. D., Gilbert, R. J. C., Watts, A., & Turberfield, A. J. (2011). DNA-templated protein arrays for single-molecule imaging. Nano Letters, 11(2), 657-660. https://doi.org/10.1021/nl1037769