DNA-templated protein arrays for single-molecule imaging

Daniele N. Selmi, Roslin J. Adamson, Helen Attrill, Alan D. Goddard, Robert J.C. Gilbert, Anthony Watts, Andrew J. Turberfield

Research output: Contribution to journalLetter

Abstract

Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

Original languageEnglish
Pages (from-to)657-660
Number of pages4
JournalNano Letters
Volume11
Issue number2
Early online date10 Jan 2011
DOIs
Publication statusPublished - 9 Feb 2011

Fingerprint

DNA
deoxyribonucleic acid
proteins
Proteins
Imaging techniques
GTP-Binding Proteins
Molecules
molecules
Image processing
Throughput
Membranes
image processing
templates
Electrons
membranes
preparation
high resolution
electrons

Keywords

  • DNA templates
  • G-protein-coupled membrane receptor
  • nanoaffinity
  • nanostructure
  • single-particle electron cryomicrosopy

Cite this

Selmi, D. N., Adamson, R. J., Attrill, H., Goddard, A. D., Gilbert, R. J. C., Watts, A., & Turberfield, A. J. (2011). DNA-templated protein arrays for single-molecule imaging. Nano Letters, 11(2), 657-660. https://doi.org/10.1021/nl1037769
Selmi, Daniele N. ; Adamson, Roslin J. ; Attrill, Helen ; Goddard, Alan D. ; Gilbert, Robert J.C. ; Watts, Anthony ; Turberfield, Andrew J. / DNA-templated protein arrays for single-molecule imaging. In: Nano Letters. 2011 ; Vol. 11, No. 2. pp. 657-660.
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Selmi, DN, Adamson, RJ, Attrill, H, Goddard, AD, Gilbert, RJC, Watts, A & Turberfield, AJ 2011, 'DNA-templated protein arrays for single-molecule imaging', Nano Letters, vol. 11, no. 2, pp. 657-660. https://doi.org/10.1021/nl1037769

DNA-templated protein arrays for single-molecule imaging. / Selmi, Daniele N.; Adamson, Roslin J.; Attrill, Helen; Goddard, Alan D.; Gilbert, Robert J.C.; Watts, Anthony; Turberfield, Andrew J.

In: Nano Letters, Vol. 11, No. 2, 09.02.2011, p. 657-660.

Research output: Contribution to journalLetter

TY - JOUR

T1 - DNA-templated protein arrays for single-molecule imaging

AU - Selmi, Daniele N.

AU - Adamson, Roslin J.

AU - Attrill, Helen

AU - Goddard, Alan D.

AU - Gilbert, Robert J.C.

AU - Watts, Anthony

AU - Turberfield, Andrew J.

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Y1 - 2011/2/9

N2 - Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

AB - Single-particle electron cryomicroscopy permits structural characterization of noncrystalline protein samples, but throughput is limited by problems associated with sample preparation and image processing. Three-dimensional density maps are reconstructed from high resolution but noisy images of individual molecules. We show that self-assembled DNA nanoaffinity templates can create dense, nonoverlapping arrays of protein molecules, greatly facilitating data collection. We demonstrate this technique using a G-protein-coupled membrane receptor, a soluble G-protein, and a signaling complex of both molecules.

KW - DNA templates

KW - G-protein-coupled membrane receptor

KW - nanoaffinity

KW - nanostructure

KW - single-particle electron cryomicrosopy

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Selmi DN, Adamson RJ, Attrill H, Goddard AD, Gilbert RJC, Watts A et al. DNA-templated protein arrays for single-molecule imaging. Nano Letters. 2011 Feb 9;11(2):657-660. https://doi.org/10.1021/nl1037769