Dopamine has bidirectional effects on synaptic responses to cortical inputs in layer II of the lateral entorhinal cortex

Douglas A. Caruana, Robert E. Sorge, Jane Stewart, C. Andrew Chapman*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Dopaminergic modulation of neuronal function has been extensively studied in the prefrontal cortex, but much less is known about its effects on glutamate-mediated synaptic transmission in the entorhinal cortex. The mesocortical dopamine system innervates the superficial layers of the lateral entorhinal cortex and may therefore modulate sensory inputs to this area. In awake rats, systemic administration of the dopamine reuptake inhibitor GBR12909 (10 mg/kg, ip) enhanced extracellular dopamine levels in the entorhinal cortex and significantly facilitated field excitatory postsynaptic potentials (fEPSPs) in layer II evoked by piriform cortex stimulation. An analysis of the receptor subtypes involved in the facilitation of evoked fEPSPs was conducted using horizontal slices of lateral entorhinal cortex in vitro. The effects of 15-min bath application of dopamine on synaptic responses were bidirectional and concentration dependent. Synaptic responses were enhanced by 10 μM dopamine and suppressed by concentrations of 50 and 100 μM. The D1-receptor antagonist SCH23390 (50 μM) blocked the significant facilitation of synaptic responses induced by 10 μM dopamine and the D2-receptor antagonist sulpiride (50 μM) prevented the suppression of fEPSPs observed with higher concentrations of dopamine. We propose here that dopamine release in the lateral entorhinal cortex, acting through D1 receptors, can lead to an enhancement of the salience of sensory representations carried to this region from adjacent sensory cortices.

Original languageEnglish
Pages (from-to)3006-3015
Number of pages10
JournalJournal of Neurophysiology
Volume96
Issue number6
DOIs
Publication statusPublished - 1 Dec 2006

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Entorhinal Cortex
Dopamine
Excitatory Postsynaptic Potentials
Dopamine Uptake Inhibitors
Sulpiride
Prefrontal Cortex
Baths
Synaptic Transmission
Glutamic Acid

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title = "Dopamine has bidirectional effects on synaptic responses to cortical inputs in layer II of the lateral entorhinal cortex",
abstract = "Dopaminergic modulation of neuronal function has been extensively studied in the prefrontal cortex, but much less is known about its effects on glutamate-mediated synaptic transmission in the entorhinal cortex. The mesocortical dopamine system innervates the superficial layers of the lateral entorhinal cortex and may therefore modulate sensory inputs to this area. In awake rats, systemic administration of the dopamine reuptake inhibitor GBR12909 (10 mg/kg, ip) enhanced extracellular dopamine levels in the entorhinal cortex and significantly facilitated field excitatory postsynaptic potentials (fEPSPs) in layer II evoked by piriform cortex stimulation. An analysis of the receptor subtypes involved in the facilitation of evoked fEPSPs was conducted using horizontal slices of lateral entorhinal cortex in vitro. The effects of 15-min bath application of dopamine on synaptic responses were bidirectional and concentration dependent. Synaptic responses were enhanced by 10 μM dopamine and suppressed by concentrations of 50 and 100 μM. The D1-receptor antagonist SCH23390 (50 μM) blocked the significant facilitation of synaptic responses induced by 10 μM dopamine and the D2-receptor antagonist sulpiride (50 μM) prevented the suppression of fEPSPs observed with higher concentrations of dopamine. We propose here that dopamine release in the lateral entorhinal cortex, acting through D1 receptors, can lead to an enhancement of the salience of sensory representations carried to this region from adjacent sensory cortices.",
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Dopamine has bidirectional effects on synaptic responses to cortical inputs in layer II of the lateral entorhinal cortex. / Caruana, Douglas A.; Sorge, Robert E.; Stewart, Jane; Chapman, C. Andrew.

In: Journal of Neurophysiology, Vol. 96, No. 6, 01.12.2006, p. 3006-3015.

Research output: Contribution to journalArticle

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