Dose-dependent modulation of the T cell proteome by ascorbic acid

Melissa M. Grant, Nalini Mistry, Joseph Lunec, Helen R. Griffiths*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

To investigate the hypothesis that the micronutrient ascorbic acid can modulate the functional genome, T cells (CCRF-HSB2) were treated with ascorbic acid (up to 150 μM) for up to 24 h. Protein expression changes were assessed by two-dimensional electrophoresis. Forty-one protein spots which showed greater than two-fold expression changes were subject to identification by matrix-assisted laser desorption ionisation time of flight MS. The confirmed protein identifications were clustered into five groups; proteins were associated with signalling, carbohydrate metabolism, apoptosis, transcription and immune function. The increased expression of phosphatidylinositol transfer protein (promotes intracellular signalling) within 5 min of ascorbic acid treatment was confirmed by Western blotting. Together, these observations suggest that ascorbic acid modulates the T cell proteome in a time- and dose-dependent manner and identify molecular targets for study following antioxidant supplementation in vivo.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalBritish Journal of Nutrition
Volume97
Issue number1
DOIs
Publication statusPublished - Jan 2007

Keywords

  • ascorbic acid
  • phosphoinositol transfer protein
  • proteomics
  • t cells

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