Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

N. A. Talaikova, A. P. Popov, A. L. Kalyanov, V. P. Ryabukho, I. V. Meglinski

Research output: Contribution to journalArticle

Abstract

A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.

Original languageEnglish
Article number105601
JournalLaser Physics Letters
Volume14
Issue number10
DOIs
Publication statusPublished - 18 Sep 2017

Fingerprint

Functional assessment
Refractive index
Microscopic examination
Diffraction
erythrocytes
refractivity
microscopy
Blood
diffraction
Imaging techniques
Spatial distribution
spatial distribution
Cells

Keywords

  • cell imaging
  • diffraction phase microscopy
  • micro-interferometry
  • quantitative phase imaging
  • red blood cells
  • refractive index

Cite this

Talaikova, N. A. ; Popov, A. P. ; Kalyanov, A. L. ; Ryabukho, V. P. ; Meglinski, I. V. / Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells. In: Laser Physics Letters. 2017 ; Vol. 14, No. 10.
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Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells. / Talaikova, N. A.; Popov, A. P.; Kalyanov, A. L.; Ryabukho, V. P.; Meglinski, I. V.

In: Laser Physics Letters, Vol. 14, No. 10, 105601, 18.09.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

AU - Talaikova, N. A.

AU - Popov, A. P.

AU - Kalyanov, A. L.

AU - Ryabukho, V. P.

AU - Meglinski, I. V.

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KW - diffraction phase microscopy

KW - micro-interferometry

KW - quantitative phase imaging

KW - red blood cells

KW - refractive index

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