Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

N. A. Talaikova; A. P. Popov; A. L. Kalyanov; V. P. Ryabukho; I. V. Meglinski

doi:10.1088/1612-202X/aa7d38

Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

N. A. Talaikova
, A. P. Popov
, A. L. Kalyanov
, V. P. Ryabukho
, I. V. Meglinski

Faculty of Information Technology and Electrical Engineering
Saratov State University
University Hospital Zurich
Institute of Precise Mechanics and Control of RAS

Research output: Contribution to journal › Article › peer-review

4 Citations (SciVal)

Abstract

A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.

Original language	English
Article number	105601
Journal	Laser Physics Letters
Volume	14
Issue number	10
DOIs	https://doi.org/10.1088/1612-202X/aa7d38
Publication status	Published - 18 Sept 2017

Funding

The authors acknowledge the support provided by CIMO Foundation (CIMO Fellowship, No. TM-15-9625), as well as partial support provided by The Ministry of Science and Education of the Russian Federation, No. 3.1340.2014/K, grant project ‘Development of optical methods and instrumentation for measurements and control of structure and dynamics of biological media’, government contract No. 2014/203 and by Government of Russian Federation, Grant 074-U01. Professor Meglinski also acknowledges partial support provided by Russian Science Foundation project No.15-14-10008.

Keywords

cell imaging
diffraction phase microscopy
micro-interferometry
quantitative phase imaging
red blood cells
refractive index

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10.1088/1612-202X/aa7d38

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title = "Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells",

abstract = "A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.",

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doi = "10.1088/1612-202X/aa7d38",

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T1 - Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

AU - Talaikova, N. A.

AU - Popov, A. P.

AU - Kalyanov, A. L.

AU - Ryabukho, V. P.

AU - Meglinski, I. V.

PY - 2017/9/18

Y1 - 2017/9/18

N2 - A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.

AB - A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.

KW - cell imaging

KW - diffraction phase microscopy

KW - micro-interferometry

KW - quantitative phase imaging

KW - red blood cells

KW - refractive index

UR - https://www.scopus.com/pages/publications/85032444284

UR - https://iopscience.iop.org/article/10.1088/1612-202X/aa7d38

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DO - 10.1088/1612-202X/aa7d38

M3 - Article

AN - SCOPUS:85032444284

SN - 1612-2011

VL - 14

JO - Laser Physics Letters

JF - Laser Physics Letters

IS - 10

M1 - 105601

ER -

Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

Abstract

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Keywords

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