TY - JOUR
T1 - Dual-mode OCT/fluorescence system for monitoring the morphology and metabolism of laser-printed 3D full-thickness skin equivalents
AU - Khalid, Arooj
AU - Dremin, Viktor
AU - El-Tamer, Ayman
AU - Surnina, Maria
AU - Lancelot, Celine
AU - Rafailov, Edik
AU - Sokolovski, Sergei
N1 - Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/). Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
PY - 2024/11/1
Y1 - 2024/11/1
N2 - The 3D structure of native human skin is fundamental for studying skin health, diseases, wound healing, and for testing the safety of skin care products, as well as personalized treatments for skin conditions. Tissue regeneration, driven by tissue engineering, often involves creating full-thickness skin equivalents (FSE), which are widely used for developing both healthy and diseased skin models. In this study, we utilized human skin cell lines to create FSE. We designed high-resolution 3D scaffolds to support the growth and maturation of these skin models. Additionally, we developed and validated a cost-effective, custom-built system combining fluorescence spectroscopy (FS) and optical coherence tomography (OCT) for non-destructive analysis of the metabolism and morphology of 3D FSEs. This system proved highly sensitive in detecting fluorescence from key metabolic co-enzymes (NADH and FAD) in solutions and cell suspensions, while OCT provided adequate resolution to observe the morphology of FSEs. As a result, both the 3D FSE model and the dual-mode optical system hold significant potential for use in 3D bioprinting of biological tissues, as well as in the development of cosmetics, drugs, and in monitoring their maturation over time.
AB - The 3D structure of native human skin is fundamental for studying skin health, diseases, wound healing, and for testing the safety of skin care products, as well as personalized treatments for skin conditions. Tissue regeneration, driven by tissue engineering, often involves creating full-thickness skin equivalents (FSE), which are widely used for developing both healthy and diseased skin models. In this study, we utilized human skin cell lines to create FSE. We designed high-resolution 3D scaffolds to support the growth and maturation of these skin models. Additionally, we developed and validated a cost-effective, custom-built system combining fluorescence spectroscopy (FS) and optical coherence tomography (OCT) for non-destructive analysis of the metabolism and morphology of 3D FSEs. This system proved highly sensitive in detecting fluorescence from key metabolic co-enzymes (NADH and FAD) in solutions and cell suspensions, while OCT provided adequate resolution to observe the morphology of FSEs. As a result, both the 3D FSE model and the dual-mode optical system hold significant potential for use in 3D bioprinting of biological tissues, as well as in the development of cosmetics, drugs, and in monitoring their maturation over time.
UR - https://www.scopus.com/inward/record.url?scp=85209070748&partnerID=8YFLogxK
UR - https://opg.optica.org/boe/fulltext.cfm?uri=boe-15-11-6299&id=561365
U2 - 10.1364/BOE.510610
DO - 10.1364/BOE.510610
M3 - Article
AN - SCOPUS:85209070748
SN - 2156-7085
VL - 15
SP - 6299
EP - 6312
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 11
ER -