Dynamic evaluation of blood flow microcirculation by combined use of the laser Doppler flowmetry and high-speed videocapillaroscopy methods

Viktor Dremin*, Igor Kozlov, Mikhail Volkov, Nikita Margaryants, Andrey Potemkin, Evgeny Zherebtsov, Andrey Dunaev, Igor Gurov

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The dynamic light scattering methods are widely used in biomedical diagnostics involving evaluation of blood flow. However, there exist some difficulties in quantitative interpretation of backscattered light signals from the viewpoint of diagnostic information. This study considers the application of the high-speed videocapillaroscopy (VCS) method that provides the direct measurement of the red blood cells (RBCs) velocity into a capillary. The VCS signal presents true oscillation nature of backscattered light caused by moving RBCs. Thus, the VCS signal can be assigned as a reference one with respect to more complicated signals like in laser Doppler flowmetry (LDF). An essential correlation between blood flow velocity oscillations in a separate human capillary and the integral perfusion estimate obtained by the LDF method has been found. The observation of blood flow by the VCS method during upper arm occlusion has shown emergence of the reverse blood flow effect in capillaries that corresponds to the biological zero signal in the LDF. The reverse blood flow effect has to be taken into account in interpretation of LDF signals.

Original languageEnglish
Article numbere201800317
JournalJournal of Biophotonics
Volume12
Issue number6
Early online date12 Jan 2019
DOIs
Publication statusPublished - Jun 2019

Keywords

  • blood flow velocity oscillations
  • laser Doppler flowmetry
  • occlusion test
  • reverse blood flow
  • videocapillaroscopy
  • wavelet coherence

Fingerprint

Dive into the research topics of 'Dynamic evaluation of blood flow microcirculation by combined use of the laser Doppler flowmetry and high-speed videocapillaroscopy methods'. Together they form a unique fingerprint.

Cite this