Ear swelling test by using laser speckle imaging with a long exposure time

Vyacheslav Kalchenko*, Yuri Kuznetsov, Dina Preise, Igor Meglinski, Alon Harmelin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Laser speckle imaging with long exposure time has been applied noninvasively to visualize the immediate reaction of cutaneous vessels in mice in response to a known primary irritant and potential allergen-methyl salicylate. The compound has been used topically on the surface of the pinna and the reaction of the vascular network was examined. We demonstrate that irritantinduced acute vascular reaction can be effectively and accurately detected by laser speckle imaging technique. The current approach holds a great promise for application in routine screening of the cutaneous vascular response induced by contact agents, screenings of mouse ear swelling test, and testing the allergenic potential of new synthetic materials and healthcare pharmaceutical products.

Original languageEnglish
Article number060502
JournalJournal of Biomedical Optics
Issue number6
Publication statusPublished - 26 Jun 2014

Bibliographical note

Copyright 2014 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.


  • allergens.
  • contact irritant
  • laser speckle imaging
  • long exposure time
  • mouse ear swelling test
  • vascular network


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