Effect of a tumour-produced lipid-mobilizing factor on protein synthesis and degradation

B.S. Islam-Ali, Michael J. Tisdale*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Treatment of murine myoblasts, myotubes and tumour cells with a tumour-produced lipid mobilizing factor (LMF), caused a concentration-dependent stimulation of protein synthesis, within a 24 h period. There was no effect on cell number or [3H] thymidine incorporation, but a similar concentration-dependent stimulation of 2-deoxyglucose uptake. LMF produced an increase in intracellular cyclic AMP levels, which was linearly (r2 = 0.973) related to the increase in protein synthesis. The effect of LMF was attenuated by the adenylate cyclase inhibitor MDL12330A, and was additive with the stimulation produced by forskolin. Both propranolol (10 μM) and the specific β3-adrenergic receptor antagonist SR 59230A (10-5M), significantly reduced the stimulation of protein synthesis induced by LMF. Protein synthesis was also increased by 69% (P = 0.006) in soleus muscles of mice administered LMF, while there was a 26% decrease in protein degradation (P = 0.03). While LMF had no effect on the lysosomal enzymes, cathepsins B and L, there was a decrease in proteasome activity, as determined both by the 'chymotrypsin-like' enzyme activity, as well as expression of proteasome α-type subunits, determined by Western blotting. These results show that in addition to its lipid-mobilizing activity LMF also increases protein accumulation in skeletal muscle both by an increase in protein synthesis and a decrease in protein catabolism. © 2001 Cancer Research Campaign.

Original languageEnglish
Pages (from-to)1648-1655
Number of pages8
JournalBritish Journal of Cancer
Volume84
Issue number12
DOIs
Publication statusPublished - 15 Jun 2001

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Proteolysis
Neoplasms
Proteins
Proteasome Endopeptidase Complex
Skeletal Muscle
Cathepsin L
Cathepsin B
Adrenergic Antagonists
Myoblasts
Skeletal Muscle Fibers
Deoxyglucose
Colforsin
lipid mobilizing substance
Propranolol
Cyclic AMP
Thymidine
Cell Count
Western Blotting
Lipids
Enzymes

Bibliographical note

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

Keywords

  • lipid mobilizing factor
  • protein synthesis
  • cyclic AMP
  • b-adrenergic receptor

Cite this

Islam-Ali, B.S. ; Tisdale, Michael J. / Effect of a tumour-produced lipid-mobilizing factor on protein synthesis and degradation. In: British Journal of Cancer. 2001 ; Vol. 84, No. 12. pp. 1648-1655.
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Effect of a tumour-produced lipid-mobilizing factor on protein synthesis and degradation. / Islam-Ali, B.S.; Tisdale, Michael J.

In: British Journal of Cancer, Vol. 84, No. 12, 15.06.2001, p. 1648-1655.

Research output: Contribution to journalArticle

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