Effect of nitric oxide on apoptotic activity in the rat gastrointestinal tract

Rakhmet I. Bersimbaev*, Yelena E. Yugai, Peter J. Hanson, Igor G. Tzoy

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The effect of nitric oxide (NO) on apoptosis in the gastrointestinal mucosa was investigated. Experiments involved long-term exposure of rat gastric mucosal cells in vitro to exogenous NO delivered from the NO, donor S-nitroso-N-acetyl-penicillamine, and the effect of intravenous administration of lipopolysaccharide in vivo, in the presence and absence of the selective inhibitor of inducible NO synthase N-(3-(aminomethyl)benzyl) acetamidine (1400 W). S-nitroso-N-acetyl-penicillamine produced a dose-related inhibition of caspase 3-like activity and DNA fragmentation in isolated gastric mucosal cells. Caspase 3-like activity and DNA fragmentation in gastric, ileal and colonic mucosa were increased both 5 and 24 h after injection of lipopolysaccharide (3 mg/kg, i.v.) to rats in vivo. Administration of 1400 W (5 mg/kg, i.v.) immediately after lipopolysaccharide enhanced caspase 3-like activity and DNA fragmentation above that found with lipopolysaccharide alone. In conclusion, data obtained both in vitro and in vivo suggest that NO exerts an anti-apoptotic effect on rat gastrointestinal mucosal cells. © 2001 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)9-16
Number of pages8
JournalEuropean Journal of Pharmacology
Volume423
Issue number1
DOIs
Publication statusPublished - 29 Jun 2001

Fingerprint

Lipopolysaccharides
Gastrointestinal Tract
Nitric Oxide
DNA Fragmentation
Caspase 3
Stomach
Penicillamine
Mucous Membrane
Nitric Oxide Donors
Nitric Oxide Synthase Type II
Intravenous Administration
Apoptosis
Injections
In Vitro Techniques
N-((3-(aminomethyl)phenyl)methyl)ethanimidamide

Keywords

  • nitric oxide (NO)
  • apoptosis
  • gastrointestinal tract
  • lipopolysaccharide

Cite this

Bersimbaev, Rakhmet I. ; Yugai, Yelena E. ; Hanson, Peter J. ; Tzoy, Igor G. / Effect of nitric oxide on apoptotic activity in the rat gastrointestinal tract. In: European Journal of Pharmacology. 2001 ; Vol. 423, No. 1. pp. 9-16.
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Effect of nitric oxide on apoptotic activity in the rat gastrointestinal tract. / Bersimbaev, Rakhmet I.; Yugai, Yelena E.; Hanson, Peter J.; Tzoy, Igor G.

In: European Journal of Pharmacology, Vol. 423, No. 1, 29.06.2001, p. 9-16.

Research output: Contribution to journalArticle

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