Effect of polymorph derived oxidants on IgG in relation to rheumatoid factor binding

Helen R. Griffiths, Joseph Lunec

Research output: Contribution to journalArticle

Abstract

Immunoglobulin G from rheumatoid patients is denatured around the hinge region. This has been proposed as an explanation for the presence of circulating autoantibodies to IgG in these patients. It has previously been suggested that oxygen radicals (OR) derived from activated polymorphs may play a role in denaturation in vivo. Using sera from rheumatoid patients and age-matched controls in a modified ELISA technique, we have investigated the potential for polyclonal rheumatoid factors (RF) to bind to OR denatured IgG. Three model systems were used to generate OR in vitro: (a) purified PMN s activated by the cell surface stimulant PMA, (b) radiolysis of IgG in solution to generate specifically the superoxide radical and, in a separate system, the hydroxyl radical, (OH.), (c) purified myeloperoxide in the presence of H2O2 and halide ions. Results: 1. The binding of both IgA and IgM RF s to PMN denatured IgG increased dose dependently for seropositive sera only. 2. The OH. radical but not the superoxide radical significantly increased the binding of IgA and M RF, again only for seropositive sera. 3. The myeloperoxidase enzyme system did not increase RF binding. 4. IgG incubated with elastase was not found to be a better antigen than native IgG. These results indicate that IgG is denatured by OR released from activated PMN, thereby producing an antigen for polyclonal RF s.
Original languageEnglish
Pages (from-to)148-156
Number of pages9
JournalScandinavian Journal of Rheumatology
Volume17
Issue numbers75
DOIs
Publication statusPublished - 1988

Fingerprint

Rheumatoid Factor
Oxidants
Immunoglobulin G
Reactive Oxygen Species
Superoxides
Immunoglobulin A
Serum
Antigens
Pancreatic Elastase
Hydroxyl Radical
Autoantibodies
Peroxidase
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay
Ions
Enzymes

Keywords

  • Immunoglobulin
  • rheumatoid patient
  • autoantibody
  • oxygen radical
  • polymorph
  • polyclonal rheumatoid factors

Cite this

Griffiths, Helen R. ; Lunec, Joseph. / Effect of polymorph derived oxidants on IgG in relation to rheumatoid factor binding. In: Scandinavian Journal of Rheumatology. 1988 ; Vol. 17, No. s75. pp. 148-156.
@article{b84d198802ee4cda935fd236c496a838,
title = "Effect of polymorph derived oxidants on IgG in relation to rheumatoid factor binding",
abstract = "Immunoglobulin G from rheumatoid patients is denatured around the hinge region. This has been proposed as an explanation for the presence of circulating autoantibodies to IgG in these patients. It has previously been suggested that oxygen radicals (OR) derived from activated polymorphs may play a role in denaturation in vivo. Using sera from rheumatoid patients and age-matched controls in a modified ELISA technique, we have investigated the potential for polyclonal rheumatoid factors (RF) to bind to OR denatured IgG. Three model systems were used to generate OR in vitro: (a) purified PMN s activated by the cell surface stimulant PMA, (b) radiolysis of IgG in solution to generate specifically the superoxide radical and, in a separate system, the hydroxyl radical, (OH.), (c) purified myeloperoxide in the presence of H2O2 and halide ions. Results: 1. The binding of both IgA and IgM RF s to PMN denatured IgG increased dose dependently for seropositive sera only. 2. The OH. radical but not the superoxide radical significantly increased the binding of IgA and M RF, again only for seropositive sera. 3. The myeloperoxidase enzyme system did not increase RF binding. 4. IgG incubated with elastase was not found to be a better antigen than native IgG. These results indicate that IgG is denatured by OR released from activated PMN, thereby producing an antigen for polyclonal RF s.",
keywords = "Immunoglobulin, rheumatoid patient, autoantibody, oxygen radical, polymorph, polyclonal rheumatoid factors",
author = "Griffiths, {Helen R.} and Joseph Lunec",
year = "1988",
doi = "10.3109/03009748809096756",
language = "English",
volume = "17",
pages = "148--156",
journal = "Scandinavian Journal of Rheumatology",
issn = "0301-3847",
publisher = "Taylor & Francis",
number = "s75",

}

Effect of polymorph derived oxidants on IgG in relation to rheumatoid factor binding. / Griffiths, Helen R.; Lunec, Joseph.

In: Scandinavian Journal of Rheumatology, Vol. 17, No. s75, 1988, p. 148-156.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of polymorph derived oxidants on IgG in relation to rheumatoid factor binding

AU - Griffiths, Helen R.

AU - Lunec, Joseph

PY - 1988

Y1 - 1988

N2 - Immunoglobulin G from rheumatoid patients is denatured around the hinge region. This has been proposed as an explanation for the presence of circulating autoantibodies to IgG in these patients. It has previously been suggested that oxygen radicals (OR) derived from activated polymorphs may play a role in denaturation in vivo. Using sera from rheumatoid patients and age-matched controls in a modified ELISA technique, we have investigated the potential for polyclonal rheumatoid factors (RF) to bind to OR denatured IgG. Three model systems were used to generate OR in vitro: (a) purified PMN s activated by the cell surface stimulant PMA, (b) radiolysis of IgG in solution to generate specifically the superoxide radical and, in a separate system, the hydroxyl radical, (OH.), (c) purified myeloperoxide in the presence of H2O2 and halide ions. Results: 1. The binding of both IgA and IgM RF s to PMN denatured IgG increased dose dependently for seropositive sera only. 2. The OH. radical but not the superoxide radical significantly increased the binding of IgA and M RF, again only for seropositive sera. 3. The myeloperoxidase enzyme system did not increase RF binding. 4. IgG incubated with elastase was not found to be a better antigen than native IgG. These results indicate that IgG is denatured by OR released from activated PMN, thereby producing an antigen for polyclonal RF s.

AB - Immunoglobulin G from rheumatoid patients is denatured around the hinge region. This has been proposed as an explanation for the presence of circulating autoantibodies to IgG in these patients. It has previously been suggested that oxygen radicals (OR) derived from activated polymorphs may play a role in denaturation in vivo. Using sera from rheumatoid patients and age-matched controls in a modified ELISA technique, we have investigated the potential for polyclonal rheumatoid factors (RF) to bind to OR denatured IgG. Three model systems were used to generate OR in vitro: (a) purified PMN s activated by the cell surface stimulant PMA, (b) radiolysis of IgG in solution to generate specifically the superoxide radical and, in a separate system, the hydroxyl radical, (OH.), (c) purified myeloperoxide in the presence of H2O2 and halide ions. Results: 1. The binding of both IgA and IgM RF s to PMN denatured IgG increased dose dependently for seropositive sera only. 2. The OH. radical but not the superoxide radical significantly increased the binding of IgA and M RF, again only for seropositive sera. 3. The myeloperoxidase enzyme system did not increase RF binding. 4. IgG incubated with elastase was not found to be a better antigen than native IgG. These results indicate that IgG is denatured by OR released from activated PMN, thereby producing an antigen for polyclonal RF s.

KW - Immunoglobulin

KW - rheumatoid patient

KW - autoantibody

KW - oxygen radical

KW - polymorph

KW - polyclonal rheumatoid factors

UR - https://www.tandfonline.com/doi/abs/10.3109/03009748809096756

U2 - 10.3109/03009748809096756

DO - 10.3109/03009748809096756

M3 - Article

VL - 17

SP - 148

EP - 156

JO - Scandinavian Journal of Rheumatology

JF - Scandinavian Journal of Rheumatology

SN - 0301-3847

IS - s75

ER -