Efecto del tratamiento con praziquantel sobre la actividad de la fosfatasa alcalina, fosfatasa acida, superoxido dismutasa en extractos crudos y productos de excreción-secreción de gusanos de Schistosoma mansoni: Praziquantel y enzimas de Schistosoma mansoni

Translated title of the contribution: Effect of Treatment with Praziquantel on the activity of alkaline phosphatase acid phosphatase, superoxide dismutase in Crude Extracts and Excretion-secretion Products of Schistosoma mansoni worms

Emilia E Barrios, Jesús Rodríguez, Naim Richani, Wolfan Araque, Juan F Quintana, Lissette Sanchez Aranguren

Research output: Contribution to journalArticle

Abstract

Venezuela is among South American countries affected by schistosomiasis and chemotherapy with praziquantel (PZQ) is the main control strategy. We determined the quantitative effect of treatment with PZQ on alkaline phosphatase activity (ALP), acid phosphatase (ACP) and superoxide dismutase (SOD) in soluble antigens of worms (SWAP) and excretion-secretion products (EEP) of male and female worms (SMWAPc, SFWAPc, ESPWMc and ESPWHc) or incubated with PZQ in vitro (SMWAPPZQ, SFWAPPZQ, ESPWMPZQ and ESPWHPZQ). Total proteins were determined by colorimetry, SOD and ACP by spectrophotometry and fluorometry ALP. There was higher protein concentration in the untreated worms EG, and the preparations obtained after incubation with PZQ in vitro, in the EG, an increase in ACP activity in the EG and PG prepared with non-treated worms and a decrease of such activity on the EG and treated PG. On the other hand, SOD activity, the EG showed statistical significance in the treated worms. In the PG showed the same behavior, but those differences were not statistically significant. Similarly, there was a decrease in the concentration of ALP noticeable in the EG and worm PGh treated worms relative to untreated statistically significant. In conclusion, we observed a decrease in total protein, ACP and SOD enzyme activities and concentration of ALP, and EG in PG treated worms.
Original languageSpanish
Pages (from-to)46-55
Number of pages10
JournalSalus
Volume17
Issue numberSuppl 1
Publication statusPublished - 2013

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praziquantel
Schistosoma mansoni
acid phosphatase
alkaline phosphatase
superoxide dismutase
excretion
secretion
extracts
colorimetry
schistosomiasis
fluorometry
proteins
Venezuela
drug therapy
spectroscopy
enzyme activity
antigens

Cite this

@article{9fb29cf82a98405abc8ca0b69e61eeca,
title = "Efecto del tratamiento con praziquantel sobre la actividad de la fosfatasa alcalina, fosfatasa acida, superoxido dismutasa en extractos crudos y productos de excreci{\'o}n-secreci{\'o}n de gusanos de Schistosoma mansoni: Praziquantel y enzimas de Schistosoma mansoni",
abstract = "Venezuela is among South American countries affected by schistosomiasis and chemotherapy with praziquantel (PZQ) is the main control strategy. We determined the quantitative effect of treatment with PZQ on alkaline phosphatase activity (ALP), acid phosphatase (ACP) and superoxide dismutase (SOD) in soluble antigens of worms (SWAP) and excretion-secretion products (EEP) of male and female worms (SMWAPc, SFWAPc, ESPWMc and ESPWHc) or incubated with PZQ in vitro (SMWAPPZQ, SFWAPPZQ, ESPWMPZQ and ESPWHPZQ). Total proteins were determined by colorimetry, SOD and ACP by spectrophotometry and fluorometry ALP. There was higher protein concentration in the untreated worms EG, and the preparations obtained after incubation with PZQ in vitro, in the EG, an increase in ACP activity in the EG and PG prepared with non-treated worms and a decrease of such activity on the EG and treated PG. On the other hand, SOD activity, the EG showed statistical significance in the treated worms. In the PG showed the same behavior, but those differences were not statistically significant. Similarly, there was a decrease in the concentration of ALP noticeable in the EG and worm PGh treated worms relative to untreated statistically significant. In conclusion, we observed a decrease in total protein, ACP and SOD enzyme activities and concentration of ALP, and EG in PG treated worms.",
author = "Barrios, {Emilia E} and Jes{\'u}s Rodr{\'i}guez and Naim Richani and Wolfan Araque and Quintana, {Juan F} and {Sanchez Aranguren}, Lissette",
year = "2013",
language = "Spanish",
volume = "17",
pages = "46--55",
number = "Suppl 1",

}

Efecto del tratamiento con praziquantel sobre la actividad de la fosfatasa alcalina, fosfatasa acida, superoxido dismutasa en extractos crudos y productos de excreción-secreción de gusanos de Schistosoma mansoni : Praziquantel y enzimas de Schistosoma mansoni. / Barrios, Emilia E ; Rodríguez, Jesús ; Richani, Naim; Araque, Wolfan ; Quintana, Juan F ; Sanchez Aranguren , Lissette.

In: Salus, Vol. 17, No. Suppl 1, 2013, p. 46-55.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Efecto del tratamiento con praziquantel sobre la actividad de la fosfatasa alcalina, fosfatasa acida, superoxido dismutasa en extractos crudos y productos de excreción-secreción de gusanos de Schistosoma mansoni

T2 - Praziquantel y enzimas de Schistosoma mansoni

AU - Barrios, Emilia E

AU - Rodríguez, Jesús

AU - Richani, Naim

AU - Araque, Wolfan

AU - Quintana, Juan F

AU - Sanchez Aranguren , Lissette

PY - 2013

Y1 - 2013

N2 - Venezuela is among South American countries affected by schistosomiasis and chemotherapy with praziquantel (PZQ) is the main control strategy. We determined the quantitative effect of treatment with PZQ on alkaline phosphatase activity (ALP), acid phosphatase (ACP) and superoxide dismutase (SOD) in soluble antigens of worms (SWAP) and excretion-secretion products (EEP) of male and female worms (SMWAPc, SFWAPc, ESPWMc and ESPWHc) or incubated with PZQ in vitro (SMWAPPZQ, SFWAPPZQ, ESPWMPZQ and ESPWHPZQ). Total proteins were determined by colorimetry, SOD and ACP by spectrophotometry and fluorometry ALP. There was higher protein concentration in the untreated worms EG, and the preparations obtained after incubation with PZQ in vitro, in the EG, an increase in ACP activity in the EG and PG prepared with non-treated worms and a decrease of such activity on the EG and treated PG. On the other hand, SOD activity, the EG showed statistical significance in the treated worms. In the PG showed the same behavior, but those differences were not statistically significant. Similarly, there was a decrease in the concentration of ALP noticeable in the EG and worm PGh treated worms relative to untreated statistically significant. In conclusion, we observed a decrease in total protein, ACP and SOD enzyme activities and concentration of ALP, and EG in PG treated worms.

AB - Venezuela is among South American countries affected by schistosomiasis and chemotherapy with praziquantel (PZQ) is the main control strategy. We determined the quantitative effect of treatment with PZQ on alkaline phosphatase activity (ALP), acid phosphatase (ACP) and superoxide dismutase (SOD) in soluble antigens of worms (SWAP) and excretion-secretion products (EEP) of male and female worms (SMWAPc, SFWAPc, ESPWMc and ESPWHc) or incubated with PZQ in vitro (SMWAPPZQ, SFWAPPZQ, ESPWMPZQ and ESPWHPZQ). Total proteins were determined by colorimetry, SOD and ACP by spectrophotometry and fluorometry ALP. There was higher protein concentration in the untreated worms EG, and the preparations obtained after incubation with PZQ in vitro, in the EG, an increase in ACP activity in the EG and PG prepared with non-treated worms and a decrease of such activity on the EG and treated PG. On the other hand, SOD activity, the EG showed statistical significance in the treated worms. In the PG showed the same behavior, but those differences were not statistically significant. Similarly, there was a decrease in the concentration of ALP noticeable in the EG and worm PGh treated worms relative to untreated statistically significant. In conclusion, we observed a decrease in total protein, ACP and SOD enzyme activities and concentration of ALP, and EG in PG treated worms.

UR - http://ve.scielo.org/scielo.php?script=sci_abstract&pid=S1316-71382013000400007&lng=en&nrm=iso&tlng=en

M3 - Article

VL - 17

SP - 46

EP - 55

IS - Suppl 1

ER -