Abstract
We present a report regarding the cytotoxic effects of iron-based magnetic nanoparticles conjugated with fluorodeoxyglucose (FDG-mNPs) on the viability of NCI-H727 and SH-SY5Y cancer cells. MTT assays were performed to determine cell viability in treated cancer cells grown under standard 2D culture conditions. FDG-mNP concentrations of 0.075 mg/mL, 0.15 mg/mL, and 0.3 mg/mL decreased mean cell viability of NCI-H727 cells to 92.5%, 82.9%, and 75% respectively. FDG-mNPs was also shown to have a detrimental effect on the viability of SY5Y cells: a decrease of 5.7%, 18.6%, and 36.4% was found for SY5Y cells treated with 0.075 mg/mL, 0.15 mg/mL, and 0.3 mg/mL concentrations of FDG-mNPs, respectively. When NCI-H727 and SH-SY5Y cancer cells were grown as 3D spheroids, morphology was visibly changed and the number of viable cells was decerased in spheroids treated with FDG-mNPs compared with untreated spheroids. The results of our study demonstrated that FDG-mNP has toxic effects on NCI-H7272 and SY5Y cancer cells, and we conclude that conjugated FDG-mNPs are promising in the development of clinical applications for the destruction of cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 53-66 |
| Number of pages | 14 |
| Journal | Asian Journal of Nanoscience and Materials |
| Volume | 4 |
| Issue number | 1 |
| Early online date | 19 Dec 2020 |
| DOIs | |
| Publication status | Published - 31 Jan 2021 |
Bibliographical note
Funding Information:Dr. Omer Aras who one of the authors was partially supported through the NIH/NCI Cancer Support Grant P30 CA008748 and the manuscript approved by Internal Review Board.
Publisher Copyright:
© 2021 by SPC (Sami Publishing Company),.
Keywords
- Fluorodeoxyglucose
- Magnetic nanoparticles
- NCI-H727
- SH-SY5Y
- Spheroids