Effects of glial cell line-derived neurotrophic factor on dental pulp cells

Zoe Gale, Paul R. Cooper, Ben A. Scheven

Research output: Contribution to journalArticle

Abstract

This study investigated the effects of glial cell line-derived neurotrophic factor (GDNF) on dental pulp cells (DPCs). Cultures of DPCs expressed GDNF as well as its receptors, GFRα1 and RET. Addition of recombinant GDNF to cultures in serum-containing medium did not significantly affect DPC growth; however, GDNF dose-dependently increased viable cell number under serum-free culture conditions. Live/dead, lactate dehydrogenase (LDH), and caspases-3/-7 assays demonstrated that cell death occurred under serum-free conditions, and that GDNF significantly reduced the number of dead cells by inhibiting apoptotic cell death. GDNF also stimulated cell proliferation in serum-free conditions, as assessed by the BrdU incorporation assay. The effect of GDNF was abolished in the presence of inhibitors to GFRα1 and RET suggesting receptor-mediated events. This study also demonstrated that GDNF counteracted TNFα-induced DPC cytotoxicity, suggesting that GDNF may be cytoprotective under disease conditions. In conclusion, our findings indicate that GDNF promotes cell survival and proliferation of DPCs and suggest that GDNF may play a multifunctional role in the regulation of dental pulp homeostasis.
Original languageEnglish
Pages (from-to)1240-1245
JournalJournal of dental research
Volume90
Issue number10
DOIs
Publication statusPublished - 1 Oct 2011

Bibliographical note

© Sage 2011. The final publication is available via Sage at http://dx.doi.org/10.1177/0022034511417443

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