Efficiency of direct photoinduced generation of singlet oxygen at different wavelengths, power density and exposure time of laser irradiation

Irina Makovik, Andrey Vinokurov, Andrey Dunaev, Edik Rafailov, Viktor Dremin

Research output: Contribution to journalArticlepeer-review

Abstract

This work investigates the influence of laser irradiation parameters (wavelength, power density and exposure time) on singlet oxygen (1O2) generation efficiency. Chemical trap (L-histidine) and fluorescent probe (Singlet Oxygen Sensor Green, SOSG) detection methods were used. Studies have been conducted for 1267, 1244, 1122 and 1064 nm laser wavelengths. 1267 nm had the highest efficiency of 1O2 generation, but 1064 nm demonstrated almost the same efficiency. We also observed that the 1244 nm wavelength can generate some amount of 1O2. It was demonstrated that laser exposure time can generate 1O2 more efficiently than an increase of power. Additionally, the SOSG fluorescence intensity measurements method for acute brain slices was studied. This allowed us to evaluate the approach's potential for in vivo detection of 1O2 concentrations.
Original languageEnglish
Pages (from-to)3559-3564
Number of pages6
JournalAnalyst
Volume148
Issue number15
DOIs
Publication statusPublished - 27 Jun 2023

Bibliographical note

Funding: The study was supported by the Russian Science Foundation
under the project no. 22-75-10088.
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

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