Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons

K.D. Fox, S.D. Greenhill, S. Glazewski

Research output: Contribution to conferencePoster

Abstract

Previous studies have shown that cortical layer 2/3 neurones show Hebbian forms of synaptic plasticity. The layer 4 to layer 2/3 pathway shows both LTP and LTD. LTP is absent in this pathway in mutants lacking autophosphorylation of CaMKII, as is experience dependent potentiation induced by single whisker experience. LTD occurs in this pathway in the deprived whisker's barrel-column, but can be occluded by whisker deprivation. In this study, we wished to determine whether homeostatic plasticity could also be detected in layer 2/3 neurones. We deprived C57BL/6J mice of all whiskers unilaterally for 1, 3, 7 or 14 days and measured principal whisker responses to a standard whisker deflection. Responses were depressed after a single day of whisker deprivation but returned toward baseline after 3 days in a homeostatic fashion despite continued whisker input deprivation. Principal whisker responses slightly overshot baseline values after 7 and 14 days deprivation. The homeostatic response was prevented in mice lacking synaptic scaling (C57BL/6OlaHsd, Ranson et al 2012, PNAS 109, 1311) and by administration of XPro1595 (Xencor, CA), a selective inhibitor of soluble TNF. TNF-alpha has itself been implicated in synaptic scaling (Stellwagen and Malenka 2006, Nature 440, 1054). We conclude that layer 2/3 neurones exhibit both Hebbian and homeostatic components of synaptic plasticity in the barrel cortex.
Original languageEnglish
Pages36.11 / F24
Publication statusPublished - 12 Nov 2016
EventNeuroscience 2016: Society for Neuroscience Annual Meeting 2016 - San Diego Convention Center, San Diego, CA, United States
Duration: 12 Nov 201616 Nov 2016
https://www.sfn.org/annual-meeting/neuroscience-2016

Conference

ConferenceNeuroscience 2016
Abbreviated titleSfN 2016
CountryUnited States
CitySan Diego, CA
Period12/11/1616/11/16
Internet address

Fingerprint

Vibrissae
Neurons
Neuronal Plasticity
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Inbred C57BL Mouse
Tumor Necrosis Factor-alpha

Bibliographical note

Abstract.

Keywords

  • neuroscience
  • plasticity

Cite this

Fox, K. D., Greenhill, S. D., & Glazewski, S. (2016). Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons. 36.11 / F24. Poster session presented at Neuroscience 2016, San Diego, CA, United States.
Fox, K.D. ; Greenhill, S.D. ; Glazewski, S. / Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons. Poster session presented at Neuroscience 2016, San Diego, CA, United States.
@conference{ddc6903213a44932acfad2a421b17a1e,
title = "Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons",
abstract = "Previous studies have shown that cortical layer 2/3 neurones show Hebbian forms of synaptic plasticity. The layer 4 to layer 2/3 pathway shows both LTP and LTD. LTP is absent in this pathway in mutants lacking autophosphorylation of CaMKII, as is experience dependent potentiation induced by single whisker experience. LTD occurs in this pathway in the deprived whisker's barrel-column, but can be occluded by whisker deprivation. In this study, we wished to determine whether homeostatic plasticity could also be detected in layer 2/3 neurones. We deprived C57BL/6J mice of all whiskers unilaterally for 1, 3, 7 or 14 days and measured principal whisker responses to a standard whisker deflection. Responses were depressed after a single day of whisker deprivation but returned toward baseline after 3 days in a homeostatic fashion despite continued whisker input deprivation. Principal whisker responses slightly overshot baseline values after 7 and 14 days deprivation. The homeostatic response was prevented in mice lacking synaptic scaling (C57BL/6OlaHsd, Ranson et al 2012, PNAS 109, 1311) and by administration of XPro1595 (Xencor, CA), a selective inhibitor of soluble TNF. TNF-alpha has itself been implicated in synaptic scaling (Stellwagen and Malenka 2006, Nature 440, 1054). We conclude that layer 2/3 neurones exhibit both Hebbian and homeostatic components of synaptic plasticity in the barrel cortex.",
keywords = "neuroscience, plasticity",
author = "K.D. Fox and S.D. Greenhill and S. Glazewski",
note = "Abstract.; Neuroscience 2016 : Society for Neuroscience Annual Meeting 2016, SfN 2016 ; Conference date: 12-11-2016 Through 16-11-2016",
year = "2016",
month = "11",
day = "12",
language = "English",
pages = "36.11 / F24",
url = "https://www.sfn.org/annual-meeting/neuroscience-2016",

}

Fox, KD, Greenhill, SD & Glazewski, S 2016, 'Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons' Neuroscience 2016, San Diego, CA, United States, 12/11/16 - 16/11/16, pp. 36.11 / F24.

Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons. / Fox, K.D.; Greenhill, S.D.; Glazewski, S.

2016. 36.11 / F24 Poster session presented at Neuroscience 2016, San Diego, CA, United States.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons

AU - Fox, K.D.

AU - Greenhill, S.D.

AU - Glazewski, S.

N1 - Abstract.

PY - 2016/11/12

Y1 - 2016/11/12

N2 - Previous studies have shown that cortical layer 2/3 neurones show Hebbian forms of synaptic plasticity. The layer 4 to layer 2/3 pathway shows both LTP and LTD. LTP is absent in this pathway in mutants lacking autophosphorylation of CaMKII, as is experience dependent potentiation induced by single whisker experience. LTD occurs in this pathway in the deprived whisker's barrel-column, but can be occluded by whisker deprivation. In this study, we wished to determine whether homeostatic plasticity could also be detected in layer 2/3 neurones. We deprived C57BL/6J mice of all whiskers unilaterally for 1, 3, 7 or 14 days and measured principal whisker responses to a standard whisker deflection. Responses were depressed after a single day of whisker deprivation but returned toward baseline after 3 days in a homeostatic fashion despite continued whisker input deprivation. Principal whisker responses slightly overshot baseline values after 7 and 14 days deprivation. The homeostatic response was prevented in mice lacking synaptic scaling (C57BL/6OlaHsd, Ranson et al 2012, PNAS 109, 1311) and by administration of XPro1595 (Xencor, CA), a selective inhibitor of soluble TNF. TNF-alpha has itself been implicated in synaptic scaling (Stellwagen and Malenka 2006, Nature 440, 1054). We conclude that layer 2/3 neurones exhibit both Hebbian and homeostatic components of synaptic plasticity in the barrel cortex.

AB - Previous studies have shown that cortical layer 2/3 neurones show Hebbian forms of synaptic plasticity. The layer 4 to layer 2/3 pathway shows both LTP and LTD. LTP is absent in this pathway in mutants lacking autophosphorylation of CaMKII, as is experience dependent potentiation induced by single whisker experience. LTD occurs in this pathway in the deprived whisker's barrel-column, but can be occluded by whisker deprivation. In this study, we wished to determine whether homeostatic plasticity could also be detected in layer 2/3 neurones. We deprived C57BL/6J mice of all whiskers unilaterally for 1, 3, 7 or 14 days and measured principal whisker responses to a standard whisker deflection. Responses were depressed after a single day of whisker deprivation but returned toward baseline after 3 days in a homeostatic fashion despite continued whisker input deprivation. Principal whisker responses slightly overshot baseline values after 7 and 14 days deprivation. The homeostatic response was prevented in mice lacking synaptic scaling (C57BL/6OlaHsd, Ranson et al 2012, PNAS 109, 1311) and by administration of XPro1595 (Xencor, CA), a selective inhibitor of soluble TNF. TNF-alpha has itself been implicated in synaptic scaling (Stellwagen and Malenka 2006, Nature 440, 1054). We conclude that layer 2/3 neurones exhibit both Hebbian and homeostatic components of synaptic plasticity in the barrel cortex.

KW - neuroscience

KW - plasticity

M3 - Poster

SP - 36.11 / F24

ER -

Fox KD, Greenhill SD, Glazewski S. Evidence for homeostatic and Hebbian plasticity components in cortical layer 2/3 neurons. 2016. Poster session presented at Neuroscience 2016, San Diego, CA, United States.