Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae

Nina Pettersson, Johan Hagström, Roslyn M. Bill, Stefan Hohmann*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

In this study the yeast Saccharomyces cerevisiae, which is a genetically tractable model for analysis of osmoregulation, has been used for analysis of heterologous aquaporins. Aquaporin water channels play important roles in the control of water homeostasis in individual cells and multicellular organisms. We have investigated the effects of functional expression of the mammalian aquaporins AQP1 and AQP5 and the aquaglyceroporins AQP3 and AQP9. Expression of aquaporins caused moderate growth inhibition under hyperosmotic stress, while expression of aquaglyceroporins mediated strong growth inhibition due to glycerol loss. Water transport was monitored in protoplasts, where the kinetics of bursting was influenced by presence of aquaporins but not aquaglyceroporins. We observed glycerol transport through aquaglyceroporins, but not aquaporins, in a yeast strain deficient in glycerol production, whose growth depends on glycerol inflow. In addition, a gene reporter assay allowed to indirectly monitor the effect of AQP9-mediated enhanced glycerol loss on osmoadaptation. Transport activity of certain aqua(glycero)porins was diminished by low pH or CuSO 4, suggesting that yeast can potentially be used for screening of putative aquaporin inhibitors. We conclude that yeast is a versatile system for functional studies of aquaporins, and it can be developed to screen for compounds of potential pharmacological use. © Springer-Verlag 2006.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalCurrent Genetics
Volume50
Issue number4
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Aquaporins
Saccharomyces cerevisiae
Aquaglyceroporins
Glycerol
Yeasts
Growth
Osmoregulation
Porins
Water
Protoplasts
Reporter Genes
Homeostasis
Pharmacology

Keywords

  • aquaglyceroporin
  • glycerol
  • inhibitors
  • osmoadaptation
  • water transport
  • yeast

Cite this

Pettersson, Nina ; Hagström, Johan ; Bill, Roslyn M. ; Hohmann, Stefan. / Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae. In: Current Genetics. 2006 ; Vol. 50, No. 4. pp. 247-255.
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Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae. / Pettersson, Nina; Hagström, Johan; Bill, Roslyn M.; Hohmann, Stefan.

In: Current Genetics, Vol. 50, No. 4, 10.2006, p. 247-255.

Research output: Contribution to journalArticle

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