Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Evgeny Zherebtsov; Viktor Dremin; Elena Zharkikh; Angelina Zherebtsova; Ilya Rafailov; Andrey Dunaev; Natalia Bazieva; Edik Rafailov

doi:10.1117/12.2290431

Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Evgeny Zherebtsov
, Viktor Dremin
, Elena Zharkikh
, Angelina Zherebtsova
, Ilya Rafailov
, Andrey Dunaev
, Natalia Bazieva
, Edik Rafailov

Orel State University

Research output: Chapter in Book/Published conference output › Conference publication

10 Citations (Scopus)

110 Downloads (Pure)

Abstract

To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.

Original language	English
Title of host publication	Dynamics and Fluctuations in Biomedical Photonics XV
Editors	Valery V. Tuchin, Ruikang K. Wang, Martin J. Leahy, Valery V. Tuchin, Kirill V. Larin
Publisher	Society of Photo-Optical Instrumentation Engineers (SPIE)
Pages	58
DOIs	https://doi.org/10.1117/12.2290431
Publication status	Published - 26 Feb 2018
Event	Dynamics and Fluctuations in Biomedical Photonics XV 2018 - San Francisco, United States Duration: 28 Jan 2018 → 29 Jan 2018

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10493
ISSN (Print)	1605-7422

Conference

Conference	Dynamics and Fluctuations in Biomedical Photonics XV 2018
Country/Territory	United States
City	San Francisco
Period	28/01/18 → 29/01/18

Bibliographical note

Copyright © 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Funding

European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 703145. Academyof Finland (grant 296408), RFBR according to the research project №18-02-00669 and grant of the President of the RussianFederation for state support of young Russian scientists № MK-3400.2018.8

Keywords

attenuation correction
blood content
diffuse reflectance
fluorescence measurements in vivo
Monte Carlo simulation
neural network
probe pressure

Access to Document

10.1117/12.2290431

Fibre-optic probe for fluorescence
Copyright 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Final published version, 636 KB

Cite this

Zherebtsov, E., Dremin, V., Zharkikh, E., Zherebtsova, A., Rafailov, I., Dunaev, A., Bazieva, N., & Rafailov, E. (2018). Fibre-optic probe for fluorescence diagnostics with blood influence compensation. In V. V. Tuchin, R. K. Wang, M. J. Leahy, V. V. Tuchin, & K. V. Larin (Eds.), Dynamics and Fluctuations in Biomedical Photonics XV (pp. 58). Article 104931L (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10493). Society of Photo-Optical Instrumentation Engineers (SPIE). https://doi.org/10.1117/12.2290431

Zherebtsov, Evgeny ; Dremin, Viktor ; Zharkikh, Elena et al. / Fibre-optic probe for fluorescence diagnostics with blood influence compensation. Dynamics and Fluctuations in Biomedical Photonics XV. editor / Valery V. Tuchin ; Ruikang K. Wang ; Martin J. Leahy ; Valery V. Tuchin ; Kirill V. Larin. Society of Photo-Optical Instrumentation Engineers (SPIE), 2018. pp. 58 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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abstract = "To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.",

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Zherebtsov, E, Dremin, V, Zharkikh, E, Zherebtsova, A, Rafailov, I, Dunaev, A, Bazieva, N & Rafailov, E 2018, Fibre-optic probe for fluorescence diagnostics with blood influence compensation. in VV Tuchin, RK Wang, MJ Leahy, VV Tuchin & KV Larin (eds), Dynamics and Fluctuations in Biomedical Photonics XV., 104931L, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10493, Society of Photo-Optical Instrumentation Engineers (SPIE), pp. 58, Dynamics and Fluctuations in Biomedical Photonics XV 2018, San Francisco, United States, 28/01/18. https://doi.org/10.1117/12.2290431

Fibre-optic probe for fluorescence diagnostics with blood influence compensation. / Zherebtsov, Evgeny; Dremin, Viktor; Zharkikh, Elena et al.
Dynamics and Fluctuations in Biomedical Photonics XV. ed. / Valery V. Tuchin; Ruikang K. Wang; Martin J. Leahy; Valery V. Tuchin; Kirill V. Larin. Society of Photo-Optical Instrumentation Engineers (SPIE), 2018. p. 58 104931L (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10493).

Research output: Chapter in Book/Published conference output › Conference publication

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T1 - Fibre-optic probe for fluorescence diagnostics with blood influence compensation

AU - Zherebtsov, Evgeny

AU - Dremin, Viktor

AU - Zharkikh, Elena

AU - Zherebtsova, Angelina

AU - Rafailov, Ilya

AU - Dunaev, Andrey

AU - Bazieva, Natalia

AU - Rafailov, Edik

N1 - Copyright © 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

PY - 2018/2/26

Y1 - 2018/2/26

N2 - To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.

AB - To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.

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KW - diffuse reflectance

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KW - Monte Carlo simulation

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KW - probe pressure

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Y2 - 28 January 2018 through 29 January 2018

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Zherebtsov E, Dremin V, Zharkikh E, Zherebtsova A, Rafailov I, Dunaev A et al. Fibre-optic probe for fluorescence diagnostics with blood influence compensation. In Tuchin VV, Wang RK, Leahy MJ, Tuchin VV, Larin KV, editors, Dynamics and Fluctuations in Biomedical Photonics XV. Society of Photo-Optical Instrumentation Engineers (SPIE). 2018. p. 58. 104931L. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2290431

Fibre-optic probe for fluorescence diagnostics with blood influence compensation

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