Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers

Ivan B Wall, Ryan Moseley, Duncan M Baird, David Kipling, Peter Giles, Iraj Laffafian, Patricia E Price, David W Thomas, Phil Stephens

Research output: Contribution to journalArticle

Abstract

Chronic age-related degenerative disorders, including the formation of chronic leg wounds, may occur due to aging of the stromal tissues and ensuing dysfunctional cellular responses. This study investigated the impact of environmental-driven cellular aging on wound healing by conducting a comprehensive analysis of chronic wound fibroblast (CWF) behavior in comparison with patient-matched healthy skin normal fibroblasts (NF). The dysfunctional wound healing abilities of CWF correlated with a significantly reduced proliferative life span and early onset of senescence compared with NF. However, pair-wise comparisons of telomere dynamics between NF and CWF indicated that the induction of senescence in CWF was telomere-independent. Microarray and functional analysis suggested that CWFs have a decreased ability to withstand oxidative stress, which may explain why these cells prematurely senescence. Microarray analysis revealed lower expression levels of several CXC chemokine genes (CXCL-1, -2, -3, -5, -6, -12) in CWF compared with NF (confirmed by ELISA). Functionally, this was related to impaired neutrophil chemotaxis in response to CWF-conditioned medium. Although the persistence of non-healing wounds is, in part, due to prolonged chronic inflammation and bacterial infection, our investigations show that premature fibroblast aging and an inability to correctly express a stromal address code are also implicated in the disease chronicity.

Original languageEnglish
Pages (from-to)2526-2540
Number of pages15
JournalJournal of Investigative Dermatology
Volume128
Issue number10
DOIs
Publication statusPublished - Oct 2008

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Varicose Ulcer
Leg Ulcer
Fibroblasts
Wounds and Injuries
Aptitude
Aging of materials
Cell Aging
Telomere
Microarray Analysis
Microarrays
Wound Healing
Premature Aging
CXC Chemokines
Functional analysis
Oxidative stress
Chemotaxis
Conditioned Culture Medium
Bacterial Infections
Leg
Skin

Keywords

  • Aged
  • Cell Proliferation
  • Cells, Cultured
  • Cellular Senescence
  • Chemokines, CXC/genetics
  • Chemotaxis, Leukocyte
  • Chronic Disease
  • Female
  • Fibroblasts
  • Gene Expression
  • Gene Expression Profiling
  • Humans
  • Leg Ulcer/genetics
  • Male
  • Microarray Analysis
  • Neutrophils
  • Oxidative Stress
  • Superoxides/metabolism
  • Telomere/genetics
  • Wound Healing

Cite this

Wall, I. B., Moseley, R., Baird, D. M., Kipling, D., Giles, P., Laffafian, I., ... Stephens, P. (2008). Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers. Journal of Investigative Dermatology, 128(10), 2526-2540. https://doi.org/10.1038/jid.2008.114
Wall, Ivan B ; Moseley, Ryan ; Baird, Duncan M ; Kipling, David ; Giles, Peter ; Laffafian, Iraj ; Price, Patricia E ; Thomas, David W ; Stephens, Phil. / Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers. In: Journal of Investigative Dermatology. 2008 ; Vol. 128, No. 10. pp. 2526-2540.
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abstract = "Chronic age-related degenerative disorders, including the formation of chronic leg wounds, may occur due to aging of the stromal tissues and ensuing dysfunctional cellular responses. This study investigated the impact of environmental-driven cellular aging on wound healing by conducting a comprehensive analysis of chronic wound fibroblast (CWF) behavior in comparison with patient-matched healthy skin normal fibroblasts (NF). The dysfunctional wound healing abilities of CWF correlated with a significantly reduced proliferative life span and early onset of senescence compared with NF. However, pair-wise comparisons of telomere dynamics between NF and CWF indicated that the induction of senescence in CWF was telomere-independent. Microarray and functional analysis suggested that CWFs have a decreased ability to withstand oxidative stress, which may explain why these cells prematurely senescence. Microarray analysis revealed lower expression levels of several CXC chemokine genes (CXCL-1, -2, -3, -5, -6, -12) in CWF compared with NF (confirmed by ELISA). Functionally, this was related to impaired neutrophil chemotaxis in response to CWF-conditioned medium. Although the persistence of non-healing wounds is, in part, due to prolonged chronic inflammation and bacterial infection, our investigations show that premature fibroblast aging and an inability to correctly express a stromal address code are also implicated in the disease chronicity.",
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Wall, IB, Moseley, R, Baird, DM, Kipling, D, Giles, P, Laffafian, I, Price, PE, Thomas, DW & Stephens, P 2008, 'Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers', Journal of Investigative Dermatology, vol. 128, no. 10, pp. 2526-2540. https://doi.org/10.1038/jid.2008.114

Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers. / Wall, Ivan B; Moseley, Ryan; Baird, Duncan M; Kipling, David; Giles, Peter; Laffafian, Iraj; Price, Patricia E; Thomas, David W; Stephens, Phil.

In: Journal of Investigative Dermatology, Vol. 128, No. 10, 10.2008, p. 2526-2540.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fibroblast dysfunction is a key factor in the non-healing of chronic venous leg ulcers

AU - Wall, Ivan B

AU - Moseley, Ryan

AU - Baird, Duncan M

AU - Kipling, David

AU - Giles, Peter

AU - Laffafian, Iraj

AU - Price, Patricia E

AU - Thomas, David W

AU - Stephens, Phil

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AB - Chronic age-related degenerative disorders, including the formation of chronic leg wounds, may occur due to aging of the stromal tissues and ensuing dysfunctional cellular responses. This study investigated the impact of environmental-driven cellular aging on wound healing by conducting a comprehensive analysis of chronic wound fibroblast (CWF) behavior in comparison with patient-matched healthy skin normal fibroblasts (NF). The dysfunctional wound healing abilities of CWF correlated with a significantly reduced proliferative life span and early onset of senescence compared with NF. However, pair-wise comparisons of telomere dynamics between NF and CWF indicated that the induction of senescence in CWF was telomere-independent. Microarray and functional analysis suggested that CWFs have a decreased ability to withstand oxidative stress, which may explain why these cells prematurely senescence. Microarray analysis revealed lower expression levels of several CXC chemokine genes (CXCL-1, -2, -3, -5, -6, -12) in CWF compared with NF (confirmed by ELISA). Functionally, this was related to impaired neutrophil chemotaxis in response to CWF-conditioned medium. Although the persistence of non-healing wounds is, in part, due to prolonged chronic inflammation and bacterial infection, our investigations show that premature fibroblast aging and an inability to correctly express a stromal address code are also implicated in the disease chronicity.

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KW - Female

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KW - Gene Expression

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KW - Humans

KW - Leg Ulcer/genetics

KW - Male

KW - Microarray Analysis

KW - Neutrophils

KW - Oxidative Stress

KW - Superoxides/metabolism

KW - Telomere/genetics

KW - Wound Healing

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