Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Nicholas J.W. Rattray, Waleed A. Zalloum, David Mansell, Joe Latimer, Carl H. Schwalbe, Alexander J. Blake, Elena V. Bichenkova*, Sally Freeman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

Original languageEnglish
Pages (from-to)6393-6395
Number of pages3
JournalChemical Communications
Volume48
Issue number51
Early online date9 May 2012
DOIs
Publication statusPublished - 28 Jun 2012

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