TY - JOUR
T1 - Fluorescent probe for detection of bacteria
T2 - conformational trigger upon bacterial reduction of an azo bridge
AU - Rattray, Nicholas J.W.
AU - Zalloum, Waleed A.
AU - Mansell, David
AU - Latimer, Joe
AU - Schwalbe, Carl H.
AU - Blake, Alexander J.
AU - Bichenkova, Elena V.
AU - Freeman, Sally
PY - 2012/6/28
Y1 - 2012/6/28
N2 - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.
AB - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.
UR - http://www.scopus.com/inward/record.url?scp=84862156781&partnerID=8YFLogxK
UR - http://pubs.rsc.org/en/Content/ArticleLanding/2012/CC/c2cc32521g#!divAbstract
U2 - 10.1039/c2cc32521g
DO - 10.1039/c2cc32521g
M3 - Article
AN - SCOPUS:84862156781
SN - 1359-7345
VL - 48
SP - 6393
EP - 6395
JO - Chemical Communications
JF - Chemical Communications
IS - 51
ER -