Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Nicholas J.W. Rattray; Waleed A. Zalloum; David Mansell; Joe Latimer; Carl H. Schwalbe; Alexander J. Blake; Elena V. Bichenkova; Sally Freeman

doi:10.1039/c2cc32521g

Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Nicholas J.W. Rattray
, Waleed A. Zalloum
, David Mansell
, Joe Latimer
, Carl H. Schwalbe
, Alexander J. Blake
, Elena V. Bichenkova^*
, Sally Freeman

^*Corresponding author for this work

Aston Pharmacy School

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

Original language	English
Pages (from-to)	6393-6395
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	51
Early online date	9 May 2012
DOIs	https://doi.org/10.1039/c2cc32521g
Publication status	Published - 28 Jun 2012

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title = "Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge",

abstract = "Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.",

author = "Rattray, \{Nicholas J.W.\} and Zalloum, \{Waleed A.\} and David Mansell and Joe Latimer and Schwalbe, \{Carl H.\} and Blake, \{Alexander J.\} and Bichenkova, \{Elena V.\} and Sally Freeman",

year = "2012",

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doi = "10.1039/c2cc32521g",

language = "English",

volume = "48",

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journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Fluorescent probe for detection of bacteria

T2 - conformational trigger upon bacterial reduction of an azo bridge

AU - Rattray, Nicholas J.W.

AU - Zalloum, Waleed A.

AU - Mansell, David

AU - Latimer, Joe

AU - Schwalbe, Carl H.

AU - Blake, Alexander J.

AU - Bichenkova, Elena V.

AU - Freeman, Sally

PY - 2012/6/28

Y1 - 2012/6/28

N2 - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

AB - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7- diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5- bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

UR - https://www.scopus.com/pages/publications/84862156781

UR - http://pubs.rsc.org/en/Content/ArticleLanding/2012/CC/c2cc32521g#!divAbstract

U2 - 10.1039/c2cc32521g

DO - 10.1039/c2cc32521g

M3 - Article

AN - SCOPUS:84862156781

SN - 1359-7345

VL - 48

SP - 6393

EP - 6395

JO - Chemical Communications

JF - Chemical Communications

IS - 51

ER -

Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Abstract

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